Maximizing transfection efficiency of vertically aligned silicon nanowire arrays

Roey Elnathan, Bahman Delalat, Daniel Brodoceanu, Hashim Alhmoud, Frances J. Harding, Katrin Buehler, Adrienne Nelson, Lucio Isa, Tobias Kraus, Nicolas H. Voelcker

Research output: Contribution to journalArticleResearchpeer-review

46 Citations (Scopus)

Abstract

Vertically aligned silicon nanowire (VA-SiNW) arrays are emerging as a powerful new tool for gene delivery by means of mechanical transfection. In order to utilize this tool efficiently, uncertainties around the required design parameters need to be removed. Here, a combination of nanosphere lithography and templated metal-assisted wet chemical etching is used to fabricate VA-SiNW arrays with a range of diameters, heights, and densities. This fabrication strategy allows identification of critical parameters of surface topography and consequently the design of SiNW arrays that deliver plasmid with high transfection efficiency into a diverse range of human cells whilst maintaining high cell viability. These results illuminate the cell-materials interactions that mediate VA-SiNW transfection and have the potential to transform gene therapy and underpin future treatment modalities.

Original languageEnglish
Pages (from-to)7215-7225
Number of pages11
JournalAdvanced Functional Materials
Volume25
Issue number46
DOIs
Publication statusPublished - 9 Dec 2015
Externally publishedYes

Keywords

  • gene delivery
  • human cells
  • nano-bio interfaces
  • nanomaterials
  • vertically aligned silicon nanowires

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