Maternal moderate physical training during pregnancy attenuates the effects of a low-protein diet on the impaired secretion of insulin in rats: Potential role for compensation of insulin resistance and preventing gestational diabetes mellitus

Carol Góis Leandro, Marco Fidalgo, Adriano Bento-Santos, Filippe Falcão-Tebas, Diogo Vasconcelos, Raul Manhães-De-Castro, Angelo Rafael Carpinelli, Sandro Massao Hirabara, Rui Curi

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Abstract

The effects of pregestational and gestational low-to-moderate physical training on insulin secretion in undernourished mothers were evaluated. Virgin female Wistar rats were divided into four groups as follows: control (C, n = 5); trained (T, n = 5); low-protein diet (LP, n = 5); trained with a low-protein diet (T + LP, n = 5). Trained rats ran on a treadmill over a period of 4 weeks before mate (5 days week-1 and 60 min day-1, at 65 of VO 2max). At pregnancy, the intensity and duration of the exercise were reduced. Low-protein groups were provided with an 8 casein diet, and controls were provided with a 17 casein diet. At third day after delivery, mothers and pups were killed and islets were isolated by collagenase digestion of pancreas and incubated for a further 1 h with medium containing 5.6 or 16.7 mM glucose. T mothers showed increased insulin secretion by isolated islets incubated with 16.7 mM glucose, whereas LP group showed reduced secretion of insulin by isolated islets when compared with both C and LP + T groups. Physical training before and during pregnancy attenuated the effects of a low-protein diet on the secretion of insulin, suggesting a potential role for compensation of insulin resistance and preventing gestational diabetes mellitus.

Original languageEnglish
Article number805418
JournalJournal of Biomedicine and Biotechnology
Volume2012
DOIs
Publication statusPublished - 2012
Externally publishedYes

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