Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 79 - 86 |
| Number of pages | 8 |
| Journal | Neonatology |
| Volume | 94 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2008 |
| Externally published | Yes |
Cite this
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Maternal betamethasone and chorioamnionitis induce different collagenases during lung maturation in fetal sheep. / Sweet, David; Huggett, Matthew; Warner, Jane; Moss, Timothy; Kloosterboer, Nico; Halliday, Henry; Newnham, John; Kallapur, Suhas; Jobe, Alan; Kramer, Boris.
In: Neonatology, Vol. 94, No. 2, 2008, p. 79 - 86.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Maternal betamethasone and chorioamnionitis induce different collagenases during lung maturation in fetal sheep
AU - Sweet, David
AU - Huggett, Matthew
AU - Warner, Jane
AU - Moss, Timothy
AU - Kloosterboer, Nico
AU - Halliday, Henry
AU - Newnham, John
AU - Kallapur, Suhas
AU - Jobe, Alan
AU - Kramer, Boris
PY - 2008
Y1 - 2008
N2 - BACKGROUND: Fetal lung maturation occurs after both maternal corticosteroid administration and chorioamnionitis. The effectors of this antenatally-induced lung maturation are not understood. Matrix metalloproteinases (MMPs) 2 and 9 are type-IV collagenases that can degrade alveolar basement membranes. OBJECTIVES: We hypothesized that the structural changes of lung maturation by both antenatal corticosteroid treatment and chorioamnionitis would be associated with increases in these MMPs. METHODS: 64 pregnant ewes were randomly assigned to one of four treatment groups: intra-amniotic injection of 10 mg endotoxin, maternal intramuscular injection of 0.5 mg/kg betamethasone, both treatments combined or saline-treated controls. We quantified MMP-2 which is derived from connective tissue and MMP-9 which is predominantly derived from neutrophils in fetal lung fluid of lambs after maternal corticosteroid therapy and induction of chorioamnionitis and the combination of both therapies given at 109-111 days gestational age with delivery 1, 5 or 15 days later. RESULTS: Betamethasone, endotoxin and the combined treatments increased both surfactant pool size, lung gas volume and reduced alveolar wall thickness at 15 days. MMP-2 concentration was increased after betamethasone. MMP-9 concentration increased after endotoxin-induced chorioamnionitis but decreased by the combined treatments. CONCLUSION: Lung maturation via different pathways may use different forms of collagenases for remodelling lung structure.
AB - BACKGROUND: Fetal lung maturation occurs after both maternal corticosteroid administration and chorioamnionitis. The effectors of this antenatally-induced lung maturation are not understood. Matrix metalloproteinases (MMPs) 2 and 9 are type-IV collagenases that can degrade alveolar basement membranes. OBJECTIVES: We hypothesized that the structural changes of lung maturation by both antenatal corticosteroid treatment and chorioamnionitis would be associated with increases in these MMPs. METHODS: 64 pregnant ewes were randomly assigned to one of four treatment groups: intra-amniotic injection of 10 mg endotoxin, maternal intramuscular injection of 0.5 mg/kg betamethasone, both treatments combined or saline-treated controls. We quantified MMP-2 which is derived from connective tissue and MMP-9 which is predominantly derived from neutrophils in fetal lung fluid of lambs after maternal corticosteroid therapy and induction of chorioamnionitis and the combination of both therapies given at 109-111 days gestational age with delivery 1, 5 or 15 days later. RESULTS: Betamethasone, endotoxin and the combined treatments increased both surfactant pool size, lung gas volume and reduced alveolar wall thickness at 15 days. MMP-2 concentration was increased after betamethasone. MMP-9 concentration increased after endotoxin-induced chorioamnionitis but decreased by the combined treatments. CONCLUSION: Lung maturation via different pathways may use different forms of collagenases for remodelling lung structure.
UR - http://content.karger.com/produktedb/produkte.asp?DOI=000115949&typ=pdf
U2 - 10.1159/000115949
DO - 10.1159/000115949
M3 - Article
VL - 94
SP - 79
EP - 86
JO - Neonatology
JF - Neonatology
SN - 1661-7800
IS - 2
ER -