Mastl is required for timely activation of APC/C in meiosis I and Cdk1 reactivation in meiosis II

Deepak Adhikari, M. Kasim Diril, Kiran Busayavalasa, Sanjiv Risal, Shoma Nakagawa, Rebecca Lindkvist, Yan Shen, Vincenzo Coppola, Lino Tessarollo, Nobuaki R Kudo, Philipp Kaldis, Kui Liu

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25 Citations (Scopus)


In mitosis, the Greatwall kinase (called microtubuleassociated serine/threonine kinase like [Mastl] in mammals)is essential for prometaphase entry or progression by suppressing protein phosphatase 2A (PP2A) activity. PP2A suppression in turn leads to high levels of Cdk1 substrate phosphorylation. We have used a mouse model with an oocyte-specific deletion of Mastl to show that Mastl-null oocytes resume meiosis I and reach metaphase I normally but that the onset and completion of anaphase I are delayed. Moreover, after the completion of meiosis I, Mastl-null oocytes failed to enter meiosis II (MII) because they reassembled a nuclear structure containing decondensed chromatin. Our results show that Mastl is required for the timely activation of anaphase-promoting complex/cyclosome to allow meiosis I exit and for the rapid rise of Cdk1 activity that is needed for the entry into MII in mouse oocytes.

Original languageEnglish
Pages (from-to)843-853
Number of pages11
JournalJournal of Cell Biology
Issue number7
Publication statusPublished - 29 Sep 2014
Externally publishedYes

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