Projects per year
Abstract
Mast cells (MCs) are important cellular components of the tumor microenvironment and are significantly associated with poor patient outcomes in prostate cancer and other solid cancers. The promotion of tumor progression partly involves heterotypic interactions between MCs and cancer‐associated fibroblasts (CAFs), which combine to potentiate a pro‐tumor extracellular matrix and promote epithelial cell invasion and migration. Thus far, the interactions between MCs and CAFs remain poorly understood. To identify molecular changes that may alter resident MC function in the prostate tumor microenvironment, we profiled the transcriptome of human prostate MCs isolated from patient‐matched non‐tumor and tumor‐associated regions of fresh radical prostatectomy tissue. Transcriptomic profiling revealed a distinct gene expression profile of MCs isolated from prostate tumor regions, including the downregulation of SAMD14, a putative tumor suppressor gene. Proteomic profiling revealed that overexpression of SAMD14 in HMC‐1 altered the secretion of proteins associated with immune regulation and extracellular matrix processes. To assess MC biological function within a model of the prostate tumor microenvironment, HMC‐1‐ SAMD14+ conditioned media was added to co‐cultures of primary prostatic CAFs and prostate epithelium. HMC‐1‐SAMD14+ secretions were shown to reduce the deposition and alignment of matrix produced by CAFs and suppress pro‐tumorigenic prostate epithelial morphology. Overall, our data present the first profile of human MCs derived from prostate cancer patient specimens and identifies MC‐derived SAMD14 as an important mediator of MC phenotype and function within the prostate tumor microenvironment.
Original language | English |
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Article number | 1237 |
Number of pages | 23 |
Journal | Cancers |
Volume | 13 |
Issue number | 6 |
DOIs | |
Publication status | Published - 2 Mar 2021 |
Keywords
- Cancer‐associated fibroblasts
- Extracellular matrix
- Mast cells
- Prostate cancer
- SAMD14
- Tumor microenvironment
Projects
- 1 Finished
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NHMRC Research Fellowship
National Health and Medical Research Council (NHMRC) (Australia)
9/03/01 → 31/12/20
Project: Research
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Equipment
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Bioinformatics Platform
Deanna Deveson (Manager)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility
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FlowCore
Andrew Fryga (Manager)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility
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Histology Platform
Camilla Cohen (Manager)
Faculty of Medicine Nursing and Health Sciences Research PlatformsFacility/equipment: Facility