Mapping biological composition through quantitative phase and absorption X-ray ptychography

Michael W.M. Jones, Kirstin Elgass, Mark D. Junker, Mac B. Luu, Michael T. Ryan, Andrew G. Peele, Grant A Van Riessen

Research output: Contribution to journalArticleResearchpeer-review

8 Citations (Scopus)

Abstract

Isolating compositional information in biological X-ray imaging can be problematic as such information is conflated with thickness and density variations when viewing in projection through a sample. We demonstrate an effective method for identifying variations in material composition by simultaneously using the quantitative phase and magnitude images provided through soft X-ray ptychography. Using this approach we show significantly increased contrast and improved reliability of the identification of intracellular features from uncharacterised samples. While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered.

Original languageEnglish
Article number6796
Number of pages4
JournalScientific Reports
Volume4
DOIs
Publication statusPublished - 28 Oct 2014
Externally publishedYes

Keywords

  • imaging techniques
  • phase-contrast microscoy
  • x-rays

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