Mapping biological composition through quantitative phase and absorption X-ray ptychography

Michael W.M. Jones; Kirstin Elgass; Mark D. Junker; Mac B. Luu; Michael T. Ryan; Andrew G. Peele; Grant A Van Riessen

doi:10.1038/srep06796

Mapping biological composition through quantitative phase and absorption X-ray ptychography

Michael W.M. Jones, Kirstin Elgass, Mark D. Junker, Mac B. Luu, Michael T. Ryan, Andrew G. Peele, Grant A Van Riessen

Research output: Contribution to journal › Article › Research › peer-review

15 Citations (Scopus)

Abstract

Isolating compositional information in biological X-ray imaging can be problematic as such information is conflated with thickness and density variations when viewing in projection through a sample. We demonstrate an effective method for identifying variations in material composition by simultaneously using the quantitative phase and magnitude images provided through soft X-ray ptychography. Using this approach we show significantly increased contrast and improved reliability of the identification of intracellular features from uncharacterised samples. While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered.

Original language	English
Article number	6796
Number of pages	4
Journal	Scientific Reports
Volume	4
DOIs	https://doi.org/10.1038/srep06796
Publication status	Published - 28 Oct 2014
Externally published	Yes

Keywords

imaging techniques
phase-contrast microscoy
x-rays

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10.1038/srep06796

40230573-oaFinal published version, 517 KB

Cite this

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title = "Mapping biological composition through quantitative phase and absorption X-ray ptychography",

abstract = "Isolating compositional information in biological X-ray imaging can be problematic as such information is conflated with thickness and density variations when viewing in projection through a sample. We demonstrate an effective method for identifying variations in material composition by simultaneously using the quantitative phase and magnitude images provided through soft X-ray ptychography. Using this approach we show significantly increased contrast and improved reliability of the identification of intracellular features from uncharacterised samples. While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered.",

keywords = "imaging techniques, phase-contrast microscoy, x-rays",

author = "Jones, {Michael W.M.} and Kirstin Elgass and Junker, {Mark D.} and Luu, {Mac B.} and Ryan, {Michael T.} and Peele, {Andrew G.} and {Van Riessen}, {Grant A}",

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doi = "10.1038/srep06796",

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AU - Elgass, Kirstin

AU - Junker, Mark D.

AU - Luu, Mac B.

AU - Ryan, Michael T.

AU - Peele, Andrew G.

AU - Van Riessen, Grant A

PY - 2014/10/28

Y1 - 2014/10/28

N2 - Isolating compositional information in biological X-ray imaging can be problematic as such information is conflated with thickness and density variations when viewing in projection through a sample. We demonstrate an effective method for identifying variations in material composition by simultaneously using the quantitative phase and magnitude images provided through soft X-ray ptychography. Using this approach we show significantly increased contrast and improved reliability of the identification of intracellular features from uncharacterised samples. While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered.

AB - Isolating compositional information in biological X-ray imaging can be problematic as such information is conflated with thickness and density variations when viewing in projection through a sample. We demonstrate an effective method for identifying variations in material composition by simultaneously using the quantitative phase and magnitude images provided through soft X-ray ptychography. Using this approach we show significantly increased contrast and improved reliability of the identification of intracellular features from uncharacterised samples. While demonstrated for X-ray ptychography, this method is immediately applicable to electron and optical microscopy methods where the complex transmission function of the sample is recovered.

KW - imaging techniques

KW - phase-contrast microscoy

KW - x-rays

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JO - Scientific Reports

JF - Scientific Reports

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ER -

Mapping biological composition through quantitative phase and absorption X-ray ptychography

Abstract

Keywords

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Australian Synchrotron

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Mapping biological composition through quantitative phase and absorption X-ray ptychography

Abstract

Keywords

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Australian Synchrotron

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