Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae

Hsing Ju Wu, Kate L. Seib, Yogitha N. Srikhanta, Jennifer Edwards, Stephen P. Kidd, Tina L. Maguire, Amanda Hamilton, Kuan Tin Pan, He Hsuan Hsiao, Chen Wen Yao, Sean M. Grimmond, Michael A. Apicella, Alastair G. McEwan, Andrew H.J. Wang, Michael P. Jennings

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24 Citations (Scopus)

Abstract

Neisseria gonorrhoeae has evolved a complex and novel network of oxidative stress responses, including defence mechanisms that are dependent on manganese (Mn). We performed systematic analyses at the transcriptomic and proteomic (1D SDS-PAGE and Isotope-Coded Affinity Tag [ICAT]) levels to investigate the global expression changes that take place in a high Mn environment, which results in a Mn-dependent oxidative stress resistance phenotype. These studies revealed that there were proteins regulated at the post-transcriptional level under conditions of increased Mn concentration, including proteins involved in virulence (e.g., pilin, a key adhesin), oxidative stress defence (e.g., superoxide dismutase), cellular metabolism, protein synthesis, RNA processing and cell division. Mn regulation of inorganic pyrophosphatase (Ppa) indicated the potential involvement of phosphate metabolism in the Mn-dependent oxidative stress defence. A detailed analysis of the role of Ppa and polyphosphate kinase (Ppk) in the gonococcal oxidative stress response revealed that ppk and ppa mutant strains showed increased resistance to oxidative stress. Investigation of these mutants grown with high Mn suggests that phosphate and pyrophosphate are involved in Mn-dependent oxidative stress resistance.

Original languageEnglish
Pages (from-to)899-916
Number of pages18
JournalJournal of Proteomics
Volume73
Issue number5
DOIs
Publication statusPublished - 10 Mar 2010
Externally publishedYes

Keywords

  • Manganese
  • Microarray
  • Neisseria gonorrhoeae
  • Oxidative stress
  • Pili
  • Proteomics

Cite this

Wu, H. J., Seib, K. L., Srikhanta, Y. N., Edwards, J., Kidd, S. P., Maguire, T. L., Hamilton, A., Pan, K. T., Hsiao, H. H., Yao, C. W., Grimmond, S. M., Apicella, M. A., McEwan, A. G., Wang, A. H. J., & Jennings, M. P. (2010). Manganese regulation of virulence factors and oxidative stress resistance in Neisseria gonorrhoeae. Journal of Proteomics, 73(5), 899-916. https://doi.org/10.1016/j.jprot.2009.12.001