Malate biosensors for the monitoring of malolactic fermentation: Different approaches

A. M. Gurban; B. Prieto-Simón; J. L. Marty; T. Noguer

doi:10.1080/00032710600713214

Malate biosensors for the monitoring of malolactic fermentation: Different approaches

A. M. Gurban, B. Prieto-Simón, J. L. Marty, T. Noguer

Research output: Contribution to journal › Article › Research › peer-review

21 Citations (Scopus)

Abstract

Different strategies for L-malic acid monitoring in wine are presented in this paper. A bi-enzymatic sensor was described based on the coupling of L-malate dehydrogenase with NADHoxidase, while monoenzymatic systems were based either on L-malate dehydrogenase or malate-quinone oxidoreductase. The bienzymatic sensors showed sensitivities of 3.6 mA/M and a detection limit of 4.5 μM, while for the monoenzymatic sensors the sensitivity was 1.1 mA/M and the detection limit was 2.4 μM. The optimized biosensors were used for determination of L-malic acid in different samples of wines.

Original language	English
Pages (from-to)	1543-1558
Number of pages	16
Journal	Analytical Letters
Volume	39
Issue number	8
DOIs	https://doi.org/10.1080/00032710600713214
Publication status	Published - 2006
Externally published	Yes

Keywords

Biosensor
Malate dehydrogenase
Malate-quinone oxidoreductase
NADHoxidase

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10.1080/00032710600713214

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title = "Malate biosensors for the monitoring of malolactic fermentation: Different approaches",

abstract = "Different strategies for L-malic acid monitoring in wine are presented in this paper. A bi-enzymatic sensor was described based on the coupling of L-malate dehydrogenase with NADHoxidase, while monoenzymatic systems were based either on L-malate dehydrogenase or malate-quinone oxidoreductase. The bienzymatic sensors showed sensitivities of 3.6 mA/M and a detection limit of 4.5 μM, while for the monoenzymatic sensors the sensitivity was 1.1 mA/M and the detection limit was 2.4 μM. The optimized biosensors were used for determination of L-malic acid in different samples of wines.",

keywords = "Biosensor, Malate dehydrogenase, Malate-quinone oxidoreductase, NADHoxidase",

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AU - Prieto-Simón, B.

AU - Marty, J. L.

AU - Noguer, T.

PY - 2006

Y1 - 2006

N2 - Different strategies for L-malic acid monitoring in wine are presented in this paper. A bi-enzymatic sensor was described based on the coupling of L-malate dehydrogenase with NADHoxidase, while monoenzymatic systems were based either on L-malate dehydrogenase or malate-quinone oxidoreductase. The bienzymatic sensors showed sensitivities of 3.6 mA/M and a detection limit of 4.5 μM, while for the monoenzymatic sensors the sensitivity was 1.1 mA/M and the detection limit was 2.4 μM. The optimized biosensors were used for determination of L-malic acid in different samples of wines.

AB - Different strategies for L-malic acid monitoring in wine are presented in this paper. A bi-enzymatic sensor was described based on the coupling of L-malate dehydrogenase with NADHoxidase, while monoenzymatic systems were based either on L-malate dehydrogenase or malate-quinone oxidoreductase. The bienzymatic sensors showed sensitivities of 3.6 mA/M and a detection limit of 4.5 μM, while for the monoenzymatic sensors the sensitivity was 1.1 mA/M and the detection limit was 2.4 μM. The optimized biosensors were used for determination of L-malic acid in different samples of wines.

KW - Biosensor

KW - Malate dehydrogenase

KW - Malate-quinone oxidoreductase

KW - NADHoxidase

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JO - Analytical Letters

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Malate biosensors for the monitoring of malolactic fermentation: Different approaches

Abstract

Keywords

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