Malate biosensors for the monitoring of malolactic fermentation: Different approaches

A. M. Gurban, B. Prieto-Simón, J. L. Marty, T. Noguer

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19 Citations (Scopus)

Abstract

Different strategies for L-malic acid monitoring in wine are presented in this paper. A bi-enzymatic sensor was described based on the coupling of L-malate dehydrogenase with NADHoxidase, while monoenzymatic systems were based either on L-malate dehydrogenase or malate-quinone oxidoreductase. The bienzymatic sensors showed sensitivities of 3.6 mA/M and a detection limit of 4.5 μM, while for the monoenzymatic sensors the sensitivity was 1.1 mA/M and the detection limit was 2.4 μM. The optimized biosensors were used for determination of L-malic acid in different samples of wines.

Original languageEnglish
Pages (from-to)1543-1558
Number of pages16
JournalAnalytical Letters
Volume39
Issue number8
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • Biosensor
  • Malate dehydrogenase
  • Malate-quinone oxidoreductase
  • NADHoxidase

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