Major surface protein LipL32 is not required for either acute or chronic infection with Leptospira interrogans

Gerald Laurence Murray, Amporn Srikram, David Edmund Hoke, Elsio A Wunder Jr., Rebekah May Henry, Miranda Lo, Kunkun Zhang, Rasana W Sermswan, Albert I Ko, Ben Adler

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Abstract

Leptospira interrogans is responsible for leptospirosis, a zoonosis of worldwide distribution. LipL32 is the major outer membrane protein of pathogenic leptospires, accounting for up to 75 of total outer membrane protein. In recent times LipL32 has become the focus of intense study because of its surface location, dominance in the host immune response and conservation among pathogenic species. In this study, a lipL32 mutant was constructed in L. interrogans using transposon mutagenesis. The lipL32 mutant had normal morphology and growth rate when compared to wild type and was equally adherent to extracellular matrix. Protein composition of the cell membranes was found to be largely unaffected by the loss of LipL32, with no obvious compensatory increase in other proteins. Microarray studies found no obvious stress response or up regulation of genes that may compensate for the loss of LipL32, but did suggest an association between LipL32 and the synthesis of heme and vitamin B12. When hamsters were inoculated by systemic and mucosal routes, the mutant caused acute severe disease manifestations which were indistinguishable from wild type L. interrogans. In the rat model of chronic infection, the LipL32 mutant colonised the renal tubules as efficiently as the wild type strain. In conclusion, this study showed that LipL32 does not play a role in either the acute or chronic models of infection. Considering the abundance and conservation of LipL32 amongst all pathogenic Leptospira spp. and its absence in saprophytic Leptospira, this finding is remarkable. The role of this protein in leptospiral biology and pathogenesis thus remains elusive.
Original languageEnglish
Pages (from-to)952 - 958
Number of pages7
JournalInfection and Immunity
Volume77
Issue number3
DOIs
Publication statusPublished - 2009

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