TY - JOUR
T1 - Major outer membrane proteins from many Campylobacter species cross-react with cholera toxin
AU - Albert, Manuel John
AU - Haridas, Shilpa
AU - Adler, Ben
PY - 2008
Y1 - 2008
N2 - We have previously shown that Campylobacter jejuni strains do not produce a functional cholera toxin-like toxin (CTLT) detectable in Chinese hamster ovary (CHO) cell assay. Instead, the 53-kDa molecular weight, major outer membrane protein (OMP), PorA of C. jejuni reacted with cholera toxin (CT) antibody in immunoblot. We extend this observation to other species of Campylobacter including C. coli, C. lari, C. fetus, C. hyointestinalis and C. upsaliensis, in which the common 53-kDa OMP reacted with CT antibody by immunoblot. There were additional reactive bands in C. fetus. As with C. jejuni, this would lead to the erroneous conclusion that these additional species also produce a functional CTLT. However, this common cross-reactive OMP can be explored as a vaccine candidate to prevent campylobacteriosis.
AB - We have previously shown that Campylobacter jejuni strains do not produce a functional cholera toxin-like toxin (CTLT) detectable in Chinese hamster ovary (CHO) cell assay. Instead, the 53-kDa molecular weight, major outer membrane protein (OMP), PorA of C. jejuni reacted with cholera toxin (CT) antibody in immunoblot. We extend this observation to other species of Campylobacter including C. coli, C. lari, C. fetus, C. hyointestinalis and C. upsaliensis, in which the common 53-kDa OMP reacted with CT antibody by immunoblot. There were additional reactive bands in C. fetus. As with C. jejuni, this would lead to the erroneous conclusion that these additional species also produce a functional CTLT. However, this common cross-reactive OMP can be explored as a vaccine candidate to prevent campylobacteriosis.
UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18353926
M3 - Article
VL - 15
SP - 859
EP - 862
JO - Clinical and Vaccine Immunology
JF - Clinical and Vaccine Immunology
SN - 1556-6811
IS - 5
ER -