Major acute-phase alpha(1)protein in the rat: Structure, molecular cloning, and regulation of mRNA levels

Tim Cole, Adam Inglis, Mariko Nagashima, Gerhard Schreiber

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Rat major acute-phase alpha(1)protein (MAP) was characterized by determining its secondary structure, ligand binding and partial amino acid sequence. A cDNA clone expressing MAP and coding for the entire mature protein was isolated from a cDNA library in E. coli prepared from rat liver mRNA. By hybridization to nick translated cDNA, mRNA for MAP was found only in liver, where it increased 17-fold during acute inflammation. Constant proportions of rates of leucine incorporation into MAP over mRNA levels in liver indicated that the regulation of the synthesis of MAP is due to a change in the rate of synthesis and/or the stability of mRNA for MAP, but not the rate of its translation.

Original languageEnglish
Pages (from-to)719-724
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number2
Publication statusPublished - 31 Jan 1985
Externally publishedYes

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