TY - JOUR
T1 - Magnetic resonance imaging contrast agent targeted toward activated platelets allows in vivo detection of thrombosis and monitoring of thrombolysis
AU - Von Zur Muhlen, Constantin
AU - von Elverfeldt, Dominik
AU - Moeller, J A
AU - Choudhury, Robin P
AU - Paul, D
AU - Hagemeyer, Christoph Eugen
AU - Olschewski, Manfred
AU - Becker, Anne-Katrin
AU - Neudorfer, Irene
AU - Bassler, Nicole
AU - Schwarz, Meike
AU - Bode, Christoph
AU - Peter, Karlheinz
PY - 2008
Y1 - 2008
N2 - Platelets are the key to thrombus formation and play a role in the development of atherosclerosis. Noninvasive imaging of activated platelets would be of great clinical interest. Here, we evaluate the ability of a magnetic resonance imaging (MRI) contrast agent consisting of microparticles of iron oxide (MPIOs) and a single-chain antibody targeting ligand-induced binding sites (LIBS) on activated glycoprotein IIb/IIIa to image carotid artery thrombi and atherosclerotic plaques. Methods and results - Anti-LIBS antibody or control antibody was conjugated to 1-?m MPIOs (LIBS MPIO/control MPIO). Nonocclusive mural thrombi were induced in mice with 6 ferric chloride. MRI (at 9.4 T) was performed once before and repeatedly in 12-minute-long sequences after =LIBS MPIO/control MPIO injection. After 36 minutes, a significant signal void, corresponding to MPIO accumulation, was observed with LIBS MPIOs but not control MPIOs (P
AB - Platelets are the key to thrombus formation and play a role in the development of atherosclerosis. Noninvasive imaging of activated platelets would be of great clinical interest. Here, we evaluate the ability of a magnetic resonance imaging (MRI) contrast agent consisting of microparticles of iron oxide (MPIOs) and a single-chain antibody targeting ligand-induced binding sites (LIBS) on activated glycoprotein IIb/IIIa to image carotid artery thrombi and atherosclerotic plaques. Methods and results - Anti-LIBS antibody or control antibody was conjugated to 1-?m MPIOs (LIBS MPIO/control MPIO). Nonocclusive mural thrombi were induced in mice with 6 ferric chloride. MRI (at 9.4 T) was performed once before and repeatedly in 12-minute-long sequences after =LIBS MPIO/control MPIO injection. After 36 minutes, a significant signal void, corresponding to MPIO accumulation, was observed with LIBS MPIOs but not control MPIOs (P
UR - http://circ.ahajournals.org/content/118/3/258
U2 - 10.1161/CIRCULATIONAHA.107.753657
DO - 10.1161/CIRCULATIONAHA.107.753657
M3 - Article
SN - 0009-7322
VL - 118
SP - 258
EP - 267
JO - Circulation
JF - Circulation
IS - 3
ER -