Magnetic Nanoparticle-Mediated Extraction and Electrochemical Detection of E. coli O157:H7 Genomic DNA

David Joram Mendoza; Ma Theresa Jonna A. Atienza-Parcon; Evangelyn C. Alocilja; Lilia M. Fernando

doi:10.1080/00032719.2024.2425665

Magnetic Nanoparticle-Mediated Extraction and Electrochemical Detection of E. coli O157:H7 Genomic DNA

David Joram Mendoza, Ma Theresa Jonna A. Atienza-Parcon, Evangelyn C. Alocilja, Lilia M. Fernando

Materials Science & Engineering

Research output: Contribution to journal › Article › Research › peer-review

1 Citation (Scopus)

Abstract

We report a rapid magnetic nanoparticle-mediated protocol to extract and detect DNA of E. coli O157:H7. Bare iron oxide nanoparticles (Fe₃O₄ NPs) were used as solid phase support to extract high purity DNA from concentrated and spiked water samples. Electrochemical detection of the DNA is achieved by employing polyaniline-functionalized magnetic nanoparticles (PANI-MNPs) which serve as DNA pre-concentrator and transducer. The DNA-based nanobiosensor shows good selectivity and sensitivity (detection limit of 0.10 ng/µL DNA) and offers faster and comparable results relative to conventional methods. This DNA extraction and detection method is expected to be applicable in food, water, and environmental samples.

Original language	English
Number of pages	16
Journal	Analytical Letters
DOIs	https://doi.org/10.1080/00032719.2024.2425665
Publication status	Accepted/In press - 9 Nov 2024

Keywords

DNA-based nanobiosensor
E. coli O157:H7
foodborne pathogen
magnetic nanoparticles
stx1 gene

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10.1080/00032719.2024.2425665

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title = "Magnetic Nanoparticle-Mediated Extraction and Electrochemical Detection of E. coli O157:H7 Genomic DNA",

abstract = "We report a rapid magnetic nanoparticle-mediated protocol to extract and detect DNA of E. coli O157:H7. Bare iron oxide nanoparticles (Fe3O4 NPs) were used as solid phase support to extract high purity DNA from concentrated and spiked water samples. Electrochemical detection of the DNA is achieved by employing polyaniline-functionalized magnetic nanoparticles (PANI-MNPs) which serve as DNA pre-concentrator and transducer. The DNA-based nanobiosensor shows good selectivity and sensitivity (detection limit of 0.10 ng/µL DNA) and offers faster and comparable results relative to conventional methods. This DNA extraction and detection method is expected to be applicable in food, water, and environmental samples.",

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T1 - Magnetic Nanoparticle-Mediated Extraction and Electrochemical Detection of E. coli O157:H7 Genomic DNA

AU - Mendoza, David Joram

AU - Atienza-Parcon, Ma Theresa Jonna A.

AU - Alocilja, Evangelyn C.

AU - Fernando, Lilia M.

PY - 2024/11/9

Y1 - 2024/11/9

N2 - We report a rapid magnetic nanoparticle-mediated protocol to extract and detect DNA of E. coli O157:H7. Bare iron oxide nanoparticles (Fe3O4 NPs) were used as solid phase support to extract high purity DNA from concentrated and spiked water samples. Electrochemical detection of the DNA is achieved by employing polyaniline-functionalized magnetic nanoparticles (PANI-MNPs) which serve as DNA pre-concentrator and transducer. The DNA-based nanobiosensor shows good selectivity and sensitivity (detection limit of 0.10 ng/µL DNA) and offers faster and comparable results relative to conventional methods. This DNA extraction and detection method is expected to be applicable in food, water, and environmental samples.

AB - We report a rapid magnetic nanoparticle-mediated protocol to extract and detect DNA of E. coli O157:H7. Bare iron oxide nanoparticles (Fe3O4 NPs) were used as solid phase support to extract high purity DNA from concentrated and spiked water samples. Electrochemical detection of the DNA is achieved by employing polyaniline-functionalized magnetic nanoparticles (PANI-MNPs) which serve as DNA pre-concentrator and transducer. The DNA-based nanobiosensor shows good selectivity and sensitivity (detection limit of 0.10 ng/µL DNA) and offers faster and comparable results relative to conventional methods. This DNA extraction and detection method is expected to be applicable in food, water, and environmental samples.

KW - DNA-based nanobiosensor

KW - E. coli O157:H7

KW - foodborne pathogen

KW - magnetic nanoparticles

KW - stx1 gene

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U2 - 10.1080/00032719.2024.2425665

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SN - 1532-236X

JO - Analytical Letters

JF - Analytical Letters

ER -

Magnetic Nanoparticle-Mediated Extraction and Electrochemical Detection of E. coli O157:H7 Genomic DNA

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Keywords

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