Magnesium sulfate reduces EEG activity but is not neuroprotective after asphyxia in preterm fetal sheep

Robert Galinsky, Vittoria Draghi, Guido Wassink, Joanne O. Davidson, Paul P. Drury, Christopher A. Lear, Alistair J. Gunn, Laura Bennet

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Magnesium sulfate is now widely recommended for neuroprotection for preterm birth; however, this has been controversial because there is little evidence that magnesium sulfate is neuroprotective. Preterm fetal sheep (104 days gestation; term is 147 days) were randomly assigned to receive sham occlusion (n=7), i.v. magnesium sulfate (n=10) or saline (n=8) starting 24 h before asphyxia until 24 h after asphyxia. Sheep were killed 72 h after asphyxia. Magnesium sulfate infusion reduced electroencephalograph power and fetal movements before asphyxia. Magnesium sulfate infusion did not affect electroencephalograph power during recovery, but was associated with marked reduction of the post-asphyxial seizure burden (mean±SD: 34±18 min vs. 107±74 min, P<0.05). Magnesium sulfate infusion did not affect subcortical neuronal loss. In the intragyral and periventricular white matter, magnesium sulfate was associated with reduced numbers of all (Olig-2+ve) oligodendrocytes in the intragyral (125±23 vs. 163±38 cells/field) and periventricular white matter (162±39 vs. 209±44 cells/field) compared to saline-treated controls (P<0.05), but no effect on microglial induction or astrogliosis. In conclusion, a clinically comparable dose of magnesium sulfate showed significant anticonvulsant effects after asphyxia in preterm fetal sheep, but did not reduce asphyxia-induced brain injury and exacerbated loss of oligodendrocytes.

Original languageEnglish
Pages (from-to)1362-1373
Number of pages12
JournalJournal of Cerebral Blood Flow and Metabolism
Volume37
Issue number4
DOIs
Publication statusPublished - 1 Apr 2017
Externally publishedYes

Keywords

  • Asphyxia
  • Brain
  • Magnesium sulfate
  • Neuroprotection
  • Preterm fetus

Cite this

Galinsky, Robert ; Draghi, Vittoria ; Wassink, Guido ; Davidson, Joanne O. ; Drury, Paul P. ; Lear, Christopher A. ; Gunn, Alistair J. ; Bennet, Laura. / Magnesium sulfate reduces EEG activity but is not neuroprotective after asphyxia in preterm fetal sheep. In: Journal of Cerebral Blood Flow and Metabolism. 2017 ; Vol. 37, No. 4. pp. 1362-1373.
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abstract = "Magnesium sulfate is now widely recommended for neuroprotection for preterm birth; however, this has been controversial because there is little evidence that magnesium sulfate is neuroprotective. Preterm fetal sheep (104 days gestation; term is 147 days) were randomly assigned to receive sham occlusion (n=7), i.v. magnesium sulfate (n=10) or saline (n=8) starting 24 h before asphyxia until 24 h after asphyxia. Sheep were killed 72 h after asphyxia. Magnesium sulfate infusion reduced electroencephalograph power and fetal movements before asphyxia. Magnesium sulfate infusion did not affect electroencephalograph power during recovery, but was associated with marked reduction of the post-asphyxial seizure burden (mean±SD: 34±18 min vs. 107±74 min, P<0.05). Magnesium sulfate infusion did not affect subcortical neuronal loss. In the intragyral and periventricular white matter, magnesium sulfate was associated with reduced numbers of all (Olig-2+ve) oligodendrocytes in the intragyral (125±23 vs. 163±38 cells/field) and periventricular white matter (162±39 vs. 209±44 cells/field) compared to saline-treated controls (P<0.05), but no effect on microglial induction or astrogliosis. In conclusion, a clinically comparable dose of magnesium sulfate showed significant anticonvulsant effects after asphyxia in preterm fetal sheep, but did not reduce asphyxia-induced brain injury and exacerbated loss of oligodendrocytes.",
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Magnesium sulfate reduces EEG activity but is not neuroprotective after asphyxia in preterm fetal sheep. / Galinsky, Robert; Draghi, Vittoria; Wassink, Guido; Davidson, Joanne O.; Drury, Paul P.; Lear, Christopher A.; Gunn, Alistair J.; Bennet, Laura.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 37, No. 4, 01.04.2017, p. 1362-1373.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Draghi, Vittoria

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