Macrophage migration inhibitory factor regulates neutrophil chemotactic responses in inflammatory arthritis in mice

Leilani Santos; Huapeng Fan; Pamela Hall; Devi Ngo; Charles Mackay; Gunter Fingerle-Rowson; Richard Bucala; Michael Hickey; Eric Morand

doi:10.1002/art.30203

Macrophage migration inhibitory factor regulates neutrophil chemotactic responses in inflammatory arthritis in mice

Leilani Santos, Huapeng Fan, Pamela Hall, Devi Ngo, Charles Mackay, Gunter Fingerle-Rowson, Richard Bucala, Michael Hickey, Eric Morand

Centre for Inflammatory Disease Monash Health

Research output: Contribution to journal › Article › Research › peer-review

75 Citations (Scopus)

Abstract

Macrophage migration inhibitory factor (MIF) facilitates multiple aspects of inflammatory arthritis, the pathogenesis of which has been significantly linked to the activity of neutrophils. The effects of MIF on neutrophil recruitment are unknown. This study was undertaken to investigate the contribution of MIF to the regulation of neutrophil chemotactic responses. METHODS: K/BxN serum-transfer arthritis was induced in wild-type (WT), MIF(-/-) , and monocyte chemotactic protein 1 (MCP-1; CCL2)-deficient mice as well as in WT mice treated with monoclonal antibodies to cytokine-induced neutrophil chemoattractant (anti-KC). Leukocyte trafficking in vivo was examined using intravital microscopy, and neutrophil function in vitro was examined using migration chambers and assessment of MAP kinase activation.

Original language	English
Pages (from-to)	960 - 970
Number of pages	11
Journal	Arthritis and Rheumatism
Volume	63
Issue number	4
DOIs	https://doi.org/10.1002/art.30203
Publication status	Published - 2011

Access to Document

10.1002/art.30203

Cite this

@article{4629c1e4e04340fda6b29b12c654a0aa,

title = "Macrophage migration inhibitory factor regulates neutrophil chemotactic responses in inflammatory arthritis in mice",

abstract = "Macrophage migration inhibitory factor (MIF) facilitates multiple aspects of inflammatory arthritis, the pathogenesis of which has been significantly linked to the activity of neutrophils. The effects of MIF on neutrophil recruitment are unknown. This study was undertaken to investigate the contribution of MIF to the regulation of neutrophil chemotactic responses. METHODS: K/BxN serum-transfer arthritis was induced in wild-type (WT), MIF(-/-) , and monocyte chemotactic protein 1 (MCP-1; CCL2)-deficient mice as well as in WT mice treated with monoclonal antibodies to cytokine-induced neutrophil chemoattractant (anti-KC). Leukocyte trafficking in vivo was examined using intravital microscopy, and neutrophil function in vitro was examined using migration chambers and assessment of MAP kinase activation.",

author = "Leilani Santos and Huapeng Fan and Pamela Hall and Devi Ngo and Charles Mackay and Gunter Fingerle-Rowson and Richard Bucala and Michael Hickey and Eric Morand",

year = "2011",

doi = "10.1002/art.30203",

language = "English",

volume = "63",

pages = "960 -- 970",

journal = "Arthritis and Rheumatism",

issn = "0004-3591",

publisher = "John Wiley & Sons",

number = "4",

}

TY - JOUR

T1 - Macrophage migration inhibitory factor regulates neutrophil chemotactic responses in inflammatory arthritis in mice

AU - Santos, Leilani

AU - Fan, Huapeng

AU - Hall, Pamela

AU - Ngo, Devi

AU - Mackay, Charles

AU - Fingerle-Rowson, Gunter

AU - Bucala, Richard

AU - Hickey, Michael

AU - Morand, Eric

PY - 2011

Y1 - 2011

N2 - Macrophage migration inhibitory factor (MIF) facilitates multiple aspects of inflammatory arthritis, the pathogenesis of which has been significantly linked to the activity of neutrophils. The effects of MIF on neutrophil recruitment are unknown. This study was undertaken to investigate the contribution of MIF to the regulation of neutrophil chemotactic responses. METHODS: K/BxN serum-transfer arthritis was induced in wild-type (WT), MIF(-/-) , and monocyte chemotactic protein 1 (MCP-1; CCL2)-deficient mice as well as in WT mice treated with monoclonal antibodies to cytokine-induced neutrophil chemoattractant (anti-KC). Leukocyte trafficking in vivo was examined using intravital microscopy, and neutrophil function in vitro was examined using migration chambers and assessment of MAP kinase activation.

AB - Macrophage migration inhibitory factor (MIF) facilitates multiple aspects of inflammatory arthritis, the pathogenesis of which has been significantly linked to the activity of neutrophils. The effects of MIF on neutrophil recruitment are unknown. This study was undertaken to investigate the contribution of MIF to the regulation of neutrophil chemotactic responses. METHODS: K/BxN serum-transfer arthritis was induced in wild-type (WT), MIF(-/-) , and monocyte chemotactic protein 1 (MCP-1; CCL2)-deficient mice as well as in WT mice treated with monoclonal antibodies to cytokine-induced neutrophil chemoattractant (anti-KC). Leukocyte trafficking in vivo was examined using intravital microscopy, and neutrophil function in vitro was examined using migration chambers and assessment of MAP kinase activation.

UR - http://onlinelibrary.wiley.com/doi/10.1002/art.30203/pdf

U2 - 10.1002/art.30203

DO - 10.1002/art.30203

M3 - Article

SN - 0004-3591

VL - 63

SP - 960

EP - 970

JO - Arthritis and Rheumatism

JF - Arthritis and Rheumatism

IS - 4

ER -

Macrophage migration inhibitory factor regulates neutrophil chemotactic responses in inflammatory arthritis in mice

Abstract

Access to Document

Other files and links

Cite this