Macrophage migration inhibitory factor regulates neutrophil chemotactic responses in inflammatory arthritis in mice

Leilani Santos, Huapeng Fan, Pamela Hall, Devi Ngo, Charles Mackay, Gunter Fingerle-Rowson, Richard Bucala, Michael Hickey, Eric Morand

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Macrophage migration inhibitory factor (MIF) facilitates multiple aspects of inflammatory arthritis, the pathogenesis of which has been significantly linked to the activity of neutrophils. The effects of MIF on neutrophil recruitment are unknown. This study was undertaken to investigate the contribution of MIF to the regulation of neutrophil chemotactic responses. METHODS: K/BxN serum-transfer arthritis was induced in wild-type (WT), MIF(-/-) , and monocyte chemotactic protein 1 (MCP-1; CCL2)-deficient mice as well as in WT mice treated with monoclonal antibodies to cytokine-induced neutrophil chemoattractant (anti-KC). Leukocyte trafficking in vivo was examined using intravital microscopy, and neutrophil function in vitro was examined using migration chambers and assessment of MAP kinase activation.
Original languageEnglish
Pages (from-to)960 - 970
Number of pages11
JournalArthritis and Rheumatism
Volume63
Issue number4
DOIs
Publication statusPublished - 2011

Cite this

@article{4629c1e4e04340fda6b29b12c654a0aa,
title = "Macrophage migration inhibitory factor regulates neutrophil chemotactic responses in inflammatory arthritis in mice",
abstract = "Macrophage migration inhibitory factor (MIF) facilitates multiple aspects of inflammatory arthritis, the pathogenesis of which has been significantly linked to the activity of neutrophils. The effects of MIF on neutrophil recruitment are unknown. This study was undertaken to investigate the contribution of MIF to the regulation of neutrophil chemotactic responses. METHODS: K/BxN serum-transfer arthritis was induced in wild-type (WT), MIF(-/-) , and monocyte chemotactic protein 1 (MCP-1; CCL2)-deficient mice as well as in WT mice treated with monoclonal antibodies to cytokine-induced neutrophil chemoattractant (anti-KC). Leukocyte trafficking in vivo was examined using intravital microscopy, and neutrophil function in vitro was examined using migration chambers and assessment of MAP kinase activation.",
author = "Leilani Santos and Huapeng Fan and Pamela Hall and Devi Ngo and Charles Mackay and Gunter Fingerle-Rowson and Richard Bucala and Michael Hickey and Eric Morand",
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doi = "10.1002/art.30203",
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journal = "Arthritis and Rheumatology",
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publisher = "Wiley-Blackwell",
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}

Macrophage migration inhibitory factor regulates neutrophil chemotactic responses in inflammatory arthritis in mice. / Santos, Leilani; Fan, Huapeng; Hall, Pamela; Ngo, Devi; Mackay, Charles; Fingerle-Rowson, Gunter; Bucala, Richard; Hickey, Michael; Morand, Eric.

In: Arthritis and Rheumatism, Vol. 63, No. 4, 2011, p. 960 - 970.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Macrophage migration inhibitory factor regulates neutrophil chemotactic responses in inflammatory arthritis in mice

AU - Santos, Leilani

AU - Fan, Huapeng

AU - Hall, Pamela

AU - Ngo, Devi

AU - Mackay, Charles

AU - Fingerle-Rowson, Gunter

AU - Bucala, Richard

AU - Hickey, Michael

AU - Morand, Eric

PY - 2011

Y1 - 2011

N2 - Macrophage migration inhibitory factor (MIF) facilitates multiple aspects of inflammatory arthritis, the pathogenesis of which has been significantly linked to the activity of neutrophils. The effects of MIF on neutrophil recruitment are unknown. This study was undertaken to investigate the contribution of MIF to the regulation of neutrophil chemotactic responses. METHODS: K/BxN serum-transfer arthritis was induced in wild-type (WT), MIF(-/-) , and monocyte chemotactic protein 1 (MCP-1; CCL2)-deficient mice as well as in WT mice treated with monoclonal antibodies to cytokine-induced neutrophil chemoattractant (anti-KC). Leukocyte trafficking in vivo was examined using intravital microscopy, and neutrophil function in vitro was examined using migration chambers and assessment of MAP kinase activation.

AB - Macrophage migration inhibitory factor (MIF) facilitates multiple aspects of inflammatory arthritis, the pathogenesis of which has been significantly linked to the activity of neutrophils. The effects of MIF on neutrophil recruitment are unknown. This study was undertaken to investigate the contribution of MIF to the regulation of neutrophil chemotactic responses. METHODS: K/BxN serum-transfer arthritis was induced in wild-type (WT), MIF(-/-) , and monocyte chemotactic protein 1 (MCP-1; CCL2)-deficient mice as well as in WT mice treated with monoclonal antibodies to cytokine-induced neutrophil chemoattractant (anti-KC). Leukocyte trafficking in vivo was examined using intravital microscopy, and neutrophil function in vitro was examined using migration chambers and assessment of MAP kinase activation.

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DO - 10.1002/art.30203

M3 - Article

VL - 63

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EP - 970

JO - Arthritis and Rheumatology

JF - Arthritis and Rheumatology

SN - 2326-5191

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