TY - JOUR
T1 - Machine-free polymerase chain reaction with triangular gold and silver nanoparticles
AU - Kadu, Pradeep
AU - Pandey, Satyaprakash
AU - Neekhra, Suditi
AU - Kumar, Rakesh
AU - Gadhe, Laxmikant
AU - Srivastava, Rohit
AU - Sastry, Murali
AU - Maji, Samir K.
PY - 2020/12/4
Y1 - 2020/12/4
N2 - Photothermal effects of metal nanoparticles (NPs) are used for various biotechnological applications. Although NPs have been used in a polymerase chain reaction (PCR), the effects of shape on the photothermal properties and its efficiency on PCR are less explored. The present study reports the synthesis of triangular gold and silver NPs, which can attain temperatures up to ∼90 °C upon irradiation with 808 nm laser. This photothermal property of synthesized nanoparticles was evaluated using various concentrations, irradiation time, and power to create a temperature profile required for variable-temperature PCR. This study reports a cost-effective, machine-free PCR using both gold and silver triangular NPs, with efficiency similar to that of a commercial PCR machine. Interestingly, addition of triangular NPs increases PCR efficiency in commercial PCR reactions. The higher PCR efficiencies are due to the direct binding and unfolding of double-stranded DNA as suggested by circular dichroism and UV spectroscopy. These findings suggest that triangular NPs can be used to develop cost-effective, robust machine-free PCR modules and can be used in various other photothermal applications.
AB - Photothermal effects of metal nanoparticles (NPs) are used for various biotechnological applications. Although NPs have been used in a polymerase chain reaction (PCR), the effects of shape on the photothermal properties and its efficiency on PCR are less explored. The present study reports the synthesis of triangular gold and silver NPs, which can attain temperatures up to ∼90 °C upon irradiation with 808 nm laser. This photothermal property of synthesized nanoparticles was evaluated using various concentrations, irradiation time, and power to create a temperature profile required for variable-temperature PCR. This study reports a cost-effective, machine-free PCR using both gold and silver triangular NPs, with efficiency similar to that of a commercial PCR machine. Interestingly, addition of triangular NPs increases PCR efficiency in commercial PCR reactions. The higher PCR efficiencies are due to the direct binding and unfolding of double-stranded DNA as suggested by circular dichroism and UV spectroscopy. These findings suggest that triangular NPs can be used to develop cost-effective, robust machine-free PCR modules and can be used in various other photothermal applications.
UR - http://www.scopus.com/inward/record.url?scp=85097763999&partnerID=8YFLogxK
U2 - 10.1021/acs.jpclett.0c02708
DO - 10.1021/acs.jpclett.0c02708
M3 - Article
C2 - 33275439
AN - SCOPUS:85097763999
VL - 11
SP - 10489
EP - 10496
JO - Journal of Physical Chemistry Letters
JF - Journal of Physical Chemistry Letters
SN - 1948-7185
IS - 24
ER -