Lysis to kill: Evaluation of the lytic abilities, and genomics of Nine bacteriophages infective for Gordonia spp. and their potential use in activated sludge foam biocontrol

Zoe A. Dyson, Joseph Tucci, Robert J. Seviour, Steve Petrovski

Research output: Contribution to journalArticleResearchpeer-review

38 Citations (Scopus)

Abstract

Nine bacteriophages (phages) infective for members of the genus Gordonia were isolated from wastewater and other natural water environments using standard enrichment techniques. The majority were broad host range phages targeting more than one Gordonia species. When their genomes were sequenced, they all emerged as double stranded DNA Siphoviridae phages, ranging from 17,562 to 103,424 bp in size, and containing between 27 and 127 genes, many of which were detailed for the first time. Many of these phage genomes diverged from the expected modular genome architecture of other characterized Siphoviridae phages and contained unusual lysis gene arrangements. Whole genome sequencing also revealed that infection with lytic phages does not appear to prevent spontaneous prophage induction in Gordonia malaquae lysogen strain BEN700. TEM sample preparation techniques were developed to view both attachment and replication stages of phage infection.

Original languageEnglish
Article numbere0134512
Number of pages16
JournalPLoS ONE
Volume10
Issue number8
DOIs
Publication statusPublished - 4 Aug 2015
Externally publishedYes

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