Lung tissue storage: Optimizing conditions for future use in molecular research

Dominic T.K. Keating, Andrea P. Malizia, D. Sadlier, C. Hurson, A. E. Wood, J. McCarthy, L. Nolke, Jim J. Egan, Peter P. Doran

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)

Abstract

The quality of tissue studied impacts greatly on oligonucleotide microarray results, emphasizing the importance of harvesting techniques. The analyzed RNA extracted from human lung samples preserved via 4 different storage conditions (RNAlater, phosphate-buffered saline, TRIzol, liquid nitrogen). RNA was assessed by denaturing gel electrophoresis, Agilent bioanalysis, real-time polymerase chain reaction (PCR), and Test3 Affymetrix chip hybridization. Results revealed better quality RNA from RNAlater samples on gel electrophoresis and bioanalysis. RNAlater samples also showed greater yield (r18s via PCR P <.05) and resulted in better Test3 chips hybridization (p <.05), suggesting RNAlater was superior at preserving lung tissue nucleic acid.

Original languageEnglish
Pages (from-to)455-466
Number of pages12
JournalExperimental Lung Research
Volume34
Issue number8
DOIs
Publication statusPublished - Oct 2008
Externally publishedYes

Keywords

  • Lung tissue
  • Nucleic acid analysis
  • RNAlater
  • Tissue storage

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