LRGUK1 is part of a multiprotein complex required for manchette function and male fertility

Hidenobu Okuda, Kathleen Deboer, Anne E. O'Connor, D. Jo Merriner, Duangporn Jamsai, Moira K. O'Bryan

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Infertility occurs in 1 in 20 young men and is idiopathic in origin in most. We have reported that the leucine-rich repeat (LRR) and guanylate kinase-like domain containing, isoform (LRGUK)-1 is essential for sperm head shaping, via the manchette, and the initiation of sperm tail growth from the centriole/basal body, and thus, male fertility. Within this study we have used a yeast 2-hybrid screen of an adult testis library to identify LRGUK1-binding partners, which were then validated with a range of techniques. The data indicate that LRGUK1 likely achieves its function in partnership with members of the HOOK family of proteins (HOOK-1–3), Rab3-interacting molecule binding protein (RIMBP)-3 and kinesin light chain (KLC)-3, all of which are associated with intracellular protein transport as cargo adaptor proteins and are localized to the manchette. LRGUK1 consists of 3 domains; an LRR, a guanylate kinase (GUK)-like and an unnamed domain. In the present study, we showed that the GUK-like domain is essential for binding to HOOK2 and RIMBP3, and the LRR domain is essential for binding to KLC3. These findings establish LRGUK1 as a key component of a multiprotein complex with an essential role in microtubule dynamics within haploid male germ cells.—Okuda, H., DeBoer, K., O’Connor, A. E., Merriner, D. J., Jamsai, D., O’Bryan, M. K. LRGUK1 is part of a multiprotein complex required for manchette function and male fertility.

Original languageEnglish
Pages (from-to)1141-1152
Number of pages12
JournalFASEB Journal
Volume31
Issue number3
DOIs
Publication statusPublished - 1 Mar 2017

Keywords

  • Cargo adaptor
  • Elongating spermatids
  • Intracellular transport
  • Sperm
  • Spermatogenesis

Cite this

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title = "LRGUK1 is part of a multiprotein complex required for manchette function and male fertility",
abstract = "Infertility occurs in 1 in 20 young men and is idiopathic in origin in most. We have reported that the leucine-rich repeat (LRR) and guanylate kinase-like domain containing, isoform (LRGUK)-1 is essential for sperm head shaping, via the manchette, and the initiation of sperm tail growth from the centriole/basal body, and thus, male fertility. Within this study we have used a yeast 2-hybrid screen of an adult testis library to identify LRGUK1-binding partners, which were then validated with a range of techniques. The data indicate that LRGUK1 likely achieves its function in partnership with members of the HOOK family of proteins (HOOK-1–3), Rab3-interacting molecule binding protein (RIMBP)-3 and kinesin light chain (KLC)-3, all of which are associated with intracellular protein transport as cargo adaptor proteins and are localized to the manchette. LRGUK1 consists of 3 domains; an LRR, a guanylate kinase (GUK)-like and an unnamed domain. In the present study, we showed that the GUK-like domain is essential for binding to HOOK2 and RIMBP3, and the LRR domain is essential for binding to KLC3. These findings establish LRGUK1 as a key component of a multiprotein complex with an essential role in microtubule dynamics within haploid male germ cells.—Okuda, H., DeBoer, K., O’Connor, A. E., Merriner, D. J., Jamsai, D., O’Bryan, M. K. LRGUK1 is part of a multiprotein complex required for manchette function and male fertility.",
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LRGUK1 is part of a multiprotein complex required for manchette function and male fertility. / Okuda, Hidenobu; Deboer, Kathleen; O'Connor, Anne E.; Merriner, D. Jo; Jamsai, Duangporn; O'Bryan, Moira K.

In: FASEB Journal, Vol. 31, No. 3, 01.03.2017, p. 1141-1152.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Okuda, Hidenobu

AU - Deboer, Kathleen

AU - O'Connor, Anne E.

AU - Merriner, D. Jo

AU - Jamsai, Duangporn

AU - O'Bryan, Moira K.

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