TY - JOUR
T1 - Low mutation rate in epaulette sharks is consistent with a slow rate of evolution in sharks
AU - Sendell-Price, Ashley T.
AU - Tulenko, Frank J.
AU - Pettersson, Mats
AU - Kang, Du
AU - Montandon, Margo
AU - Winkler, Sylke
AU - Kulb, Kathleen
AU - Naylor, Gavin P.
AU - Phillippy, Adam
AU - Fedrigo, Olivier
AU - Mountcastle, Jacquelyn
AU - Balacco, Jennifer R.
AU - Dutra, Amalia
AU - Dale, Rebecca E.
AU - Haase, Bettina
AU - Jarvis, Erich D.
AU - Myers, Gene
AU - Burgess, Shawn M.
AU - Currie, Peter D.
AU - Andersson, Leif
AU - Schartl, Manfred
N1 - Funding Information:
We gratefully acknowledge the contribution of the Long Read Team of the Dresden Concept Genome Centre. We thank M. Hof for the information and discussion. This study was supported by the DFG Research Infrastructure NGS-CC as part of the Next Generation Sequencing Competence Network (project 423957469) and grants from the Deutsche Forschungsgemeinschaft (SCHA 408/15-1) as part of the DFG Sequencing call to M.S., Vetenskapsrådet (2017-02907) and Knut and Alice Wallenberg Foundation (K.A.W. 2016.0361) to L.A., Australian Research Council Discovery Grant DP220102970 to P.D.C. and F.T., Florida Museum of Natural History to G.P.N., and an NHMRC Fellowship GNT1136567 to P.D.C. This research was supported in part by the Intramural Research Programme of the National Human Genome Research Institute (ZIAHG200386-06). The authors would like to acknowledge the use of computing resources at Uppsala Multidisciplinary Centre for Advanced Computational Science (UPPMAX) in carrying out this work.
Funding Information:
We gratefully acknowledge the contribution of the Long Read Team of the Dresden Concept Genome Centre. We thank M. Hof for the information and discussion. This study was supported by the DFG Research Infrastructure NGS-CC as part of the Next Generation Sequencing Competence Network (project 423957469) and grants from the Deutsche Forschungsgemeinschaft (SCHA 408/15-1) as part of the DFG Sequencing call to M.S., Vetenskapsrådet (2017-02907) and Knut and Alice Wallenberg Foundation (K.A.W. 2016.0361) to L.A., Australian Research Council Discovery Grant DP220102970 to P.D.C. and F.T., Florida Museum of Natural History to G.P.N., and an NHMRC Fellowship GNT1136567 to P.D.C. This research was supported in part by the Intramural Research Programme of the National Human Genome Research Institute (ZIAHG200386-06). The authors would like to acknowledge the use of computing resources at Uppsala Multidisciplinary Centre for Advanced Computational Science (UPPMAX) in carrying out this work.
Publisher Copyright:
© 2023, Springer Nature Limited.
PY - 2023/10/19
Y1 - 2023/10/19
N2 - Sharks occupy diverse ecological niches and play critical roles in marine ecosystems, often acting as apex predators. They are considered a slow-evolving lineage and have been suggested to exhibit exceptionally low cancer rates. These two features could be explained by a low nuclear mutation rate. Here, we provide a direct estimate of the nuclear mutation rate in the epaulette shark (Hemiscyllium ocellatum). We generate a high-quality reference genome, and resequence the whole genomes of parents and nine offspring to detect de novo mutations. Using stringent criteria, we estimate a mutation rate of 7×10−10 per base pair, per generation. This represents one of the lowest directly estimated mutation rates for any vertebrate clade, indicating that this basal vertebrate group is indeed a slowly evolving lineage whose ability to restore genetic diversity following a sustained population bottleneck may be hampered by a low mutation rate.
AB - Sharks occupy diverse ecological niches and play critical roles in marine ecosystems, often acting as apex predators. They are considered a slow-evolving lineage and have been suggested to exhibit exceptionally low cancer rates. These two features could be explained by a low nuclear mutation rate. Here, we provide a direct estimate of the nuclear mutation rate in the epaulette shark (Hemiscyllium ocellatum). We generate a high-quality reference genome, and resequence the whole genomes of parents and nine offspring to detect de novo mutations. Using stringent criteria, we estimate a mutation rate of 7×10−10 per base pair, per generation. This represents one of the lowest directly estimated mutation rates for any vertebrate clade, indicating that this basal vertebrate group is indeed a slowly evolving lineage whose ability to restore genetic diversity following a sustained population bottleneck may be hampered by a low mutation rate.
UR - http://www.scopus.com/inward/record.url?scp=85174520669&partnerID=8YFLogxK
U2 - 10.1038/s41467-023-42238-x
DO - 10.1038/s41467-023-42238-x
M3 - Article
C2 - 37857613
AN - SCOPUS:85174520669
SN - 2041-1723
VL - 14
JO - Nature Communications
JF - Nature Communications
IS - 1
M1 - 6628
ER -
