With 1 figure and 1 table The aim of the present study was to compare the durum and bread wheat alleles encoded at the Glu-A3 locus (represented by Glu-Ad3 and Glu-Aa3, respectively) using PCR markers designed for bread wheat Glu-Aa3 alleles to distinguish different durum alleles. Diversity in low-molecular-weight glutenin subunits (LMW-GS) was analysed in eight cultivars representing all known Glu-Ad3 alleles present in durum wheat. Six of the eight Glu-Ad3 alleles of durum wheat could be distinguished using a combination of seven different primer sets based on LMW-GS sequences from bread wheat. However, allele a (cv. 'Mexicali'), allele b (cv. 'Langdon') and allele c (cv. 'Cocorit') of durum wheat could not be distinguished using any of the markers tested. Amplicons of identical size were produced for alleles f (cv. 'Clarofino') and allele g (cv. 'Claro de Balazote'), using all except one primer set (Glu-A3a), indicating genes of these alleles share a very high degree of identity. The markers described in this study are useful for the DNA-based identification of durum wheat cultivars and the identification of the Glu-Ad3 a, b, c, d, e, f, g and h alleles of durum wheat for the purposes of marker-assisted selection.
- Glu-A3 locus
- Low-molecular-weight glutenin subunits
- PCR marker
- Triticum aestivum
- Triticum turgidum ssp durum Desf.
- Variety identification