Low fouling electrospun scaffolds with clicked bioactive peptides for specific cell attachment

Andrew E Rodda, Francesca Ercole, Veronica Glattauer, James Gardiner, David Russell Nisbet, Kevin E Healy, John Stanley Forsythe, Laurence Meagher

Research output: Contribution to journalArticleResearchpeer-review

Abstract

While electrospun fibers are of interest as scaffolds for tissue engineering applications, nonspecific surface interactions such as protein adsorption often prevent researchers from controlling the exact interactions between cells and the underlying material. In this study we prepared electrospun fibers from a polystyrene-based macroinitiator, which were then grafted with polymer brushes using surface-initiated atom transfer radical polymerization (SI-ATRP). These brush coatings incorporated a trimethylsilyl-protected PEG-alkyne monomer, allowing azide functional molecules to be covalently attached, while simultaneously reducing nonspecific protein adsorption on the fibers. Cells were able to attach and spread on fibrous substrates functionalized with a pendant RGD-containing peptide, while spreading was significantly reduced on nonfunctionalized fibers and those with the equivalent RGE control peptide. This effect was observed both in the presence and absence of serum in the culture media, indicating that protein adsorption on the fibers was minimal and cell adhesion within the fibrous scaffold was mediated almost entirely through the cell-adhesive RGD-containing peptide.
Original languageEnglish
Pages (from-to)2109 - 2118
Number of pages10
JournalBiomacromolecules
Volume16
Issue number7
DOIs
Publication statusPublished - 2015

Cite this

Rodda, Andrew E ; Ercole, Francesca ; Glattauer, Veronica ; Gardiner, James ; Nisbet, David Russell ; Healy, Kevin E ; Forsythe, John Stanley ; Meagher, Laurence. / Low fouling electrospun scaffolds with clicked bioactive peptides for specific cell attachment. In: Biomacromolecules. 2015 ; Vol. 16, No. 7. pp. 2109 - 2118.
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abstract = "While electrospun fibers are of interest as scaffolds for tissue engineering applications, nonspecific surface interactions such as protein adsorption often prevent researchers from controlling the exact interactions between cells and the underlying material. In this study we prepared electrospun fibers from a polystyrene-based macroinitiator, which were then grafted with polymer brushes using surface-initiated atom transfer radical polymerization (SI-ATRP). These brush coatings incorporated a trimethylsilyl-protected PEG-alkyne monomer, allowing azide functional molecules to be covalently attached, while simultaneously reducing nonspecific protein adsorption on the fibers. Cells were able to attach and spread on fibrous substrates functionalized with a pendant RGD-containing peptide, while spreading was significantly reduced on nonfunctionalized fibers and those with the equivalent RGE control peptide. This effect was observed both in the presence and absence of serum in the culture media, indicating that protein adsorption on the fibers was minimal and cell adhesion within the fibrous scaffold was mediated almost entirely through the cell-adhesive RGD-containing peptide.",
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Low fouling electrospun scaffolds with clicked bioactive peptides for specific cell attachment. / Rodda, Andrew E; Ercole, Francesca; Glattauer, Veronica; Gardiner, James; Nisbet, David Russell; Healy, Kevin E; Forsythe, John Stanley; Meagher, Laurence.

In: Biomacromolecules, Vol. 16, No. 7, 2015, p. 2109 - 2118.

Research output: Contribution to journalArticleResearchpeer-review

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