Longitudinal analysis of low-molecular weight fluorophores in type 1 diabetes mellitus

Andrzej S. Januszewski, Merlin C. Thomas, Connie S. Karschimkus, Jasmine S. Chung, Kevin G. Rowley, Craig L. Nelson, David N. O'Neal, George Dragicevic, Colin A. Harper, James D. Best, Alicia J. Jenkins

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4 Citations (Scopus)

Abstract

Objectives : Circulating low molecular weight (<10 kDa) fluorophores (LMW-F) measured by non-specific fluorescence spectroscopy may detect small advanced glycation end-products (AGEs) not recognized by other assays. This longitudinal study assessed correlates of LMW-F and predictive power of LMW-F levels for vascular health in Type 1 diabetes (T1DM) patients. Methods : Fasting patients with T1DM (n=37) were studied twice at intervals of 12-60 months (mean±SD, 33±15 months). LMW-F levels were also measured once in 112 healthy control subjects. Results : Relative to controls, LMW-F levels were higher in diabetic subjects at initial and final time points (mean±SD), 5.4±1.9 AU/ml and 4.5±1.8 AU/ml respectively vs. 3.8±2.1 AU/ml ; p=0.0001 and p=0.06). Baseline LMW-F levels predicted subsequent hs-CRP and oxLDL/LDL values. LMW-F levels decreased significantly over time in diabetes (5.4 ± 1.9 vs. 4.5 ± 1.8 AU/ml ; p=0.02). Rises in LMW-F levels in individual diabetic subjects correlated significantly with worsening renal function (BUN), glycemia (HbA1c) and with vascular dysfunction (systemic vascular resistance). Conclusions : LMW-F levels predict levels of inflammation and oxidation in T1DM. Changes in LMW-F levels in T1DM reflect variations in glycemia and renal function. Biochemical characterization of LMW-F would facilitate understanding of the potential utility of LMW-F as a therapeutic target.

Original languageEnglish
Pages (from-to)29-36
Number of pages8
JournalJournal of Medical Investigation
Volume55
Issue number1-2
DOIs
Publication statusPublished - 1 Feb 2008
Externally publishedYes

Keywords

  • Advanced glycation end products
  • Low molecular weight fluorophores
  • Renal function
  • Type 1 diabetes

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