Long-term pulmonary effects of intrauterine exposure to endotoxin following preterm birth in sheep

Anzari Arfana Atik, Foula Sozo, Sandra Orgeig, Lakshmi Suri, Takushi Hanita, Richard Harding, Robert M De Matteo

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6 Citations (Scopus)

Abstract

Our aim was to determine whether fetal exposure to intraamniotic lipopolysaccharide (LPS) persistently alters the lungs following moderate preterm birth. Fetal sheep were exposed to LPS (1 mg/d) or saline from 0.75 to preterm birth at 0.90 of gestation. Eleven weeks after preterm birth, lung structure was unaltered. Interleukin (IL)-1beta messenger RNA (mRNA) levels were elevated in lungs of LPS-exposed lambs (P <.05) but IL-1beta protein levels were unaltered. Lung mRNA levels of IL-6, IL-8 and tumor necrosis factor alpha, and percentage of inflammatory cells were not different between groups. Surfactant protein (SP)-A and SP-C mRNA levels and SP-B tissue protein expression were higher in LPS-exposed lambs than controls (all P <.05); however, expression of SP-A and SP-C proteins was reduced. Prenatal LPS exposure causes a persistent increase in gene expression of proinflammatory mediators and surfactant proteins and a decrease in lung tissue SP-A and -C protein expression after preterm birth, which may affect lung immunity.
Original languageEnglish
Pages (from-to)1352 - 1364
Number of pages13
JournalReproductive Sciences
Volume19
Issue number12
DOIs
Publication statusPublished - 2012

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