Long-term, inducible gene loss-of-function in the chicken embryo

Olivier Serralbo, Cyril Picard, Christophe Marcelle

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

The use of shRNAmir to down-regulate the expression of genes of interest is a powerful tool for studying gene function during early chick development. However, because of the limitations of electroporation-mediated transgenesis, the down-regulation of genes expressed at late stages of development in specific tissues is difficult to perform. By combining electroporation of a doxycycline-inducible, miR30-based shRNA plasmid with the Tol2 genomic integration system, we are now able to down-regulate the expression of any gene of interest at defined stage of chicken development. genesis 00:1-9. (c) 2013 Wiley Periodicals, Inc.
Original languageEnglish
Pages (from-to)372 - 380
Number of pages9
Journalgenesis: The Journal of Genetics and Development
Volume51
Issue number5
DOIs
Publication statusPublished - 2013

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