Long-term, inducible gene loss-of-function in the chicken embryo

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The use of shRNAmir to down-regulate the expression of genes of interest is a powerful tool for studying gene function during early chick development. However, because of the limitations of electroporation-mediated transgenesis, the down-regulation of genes expressed at late stages of development in specific tissues is difficult to perform. By combining electroporation of a doxycycline-inducible, miR30-based shRNA plasmid with the Tol2 genomic integration system, we are now able to down-regulate the expression of any gene of interest at defined stage of chicken development. genesis 00:1-9. (c) 2013 Wiley Periodicals, Inc.
Original languageEnglish
Pages (from-to)372 - 380
Number of pages9
JournalGenesis
Volume51
Issue number5
DOIs
Publication statusPublished - 2013

Cite this

@article{87f80d47289f44959f41db06871e0140,
title = "Long-term, inducible gene loss-of-function in the chicken embryo",
abstract = "The use of shRNAmir to down-regulate the expression of genes of interest is a powerful tool for studying gene function during early chick development. However, because of the limitations of electroporation-mediated transgenesis, the down-regulation of genes expressed at late stages of development in specific tissues is difficult to perform. By combining electroporation of a doxycycline-inducible, miR30-based shRNA plasmid with the Tol2 genomic integration system, we are now able to down-regulate the expression of any gene of interest at defined stage of chicken development. genesis 00:1-9. (c) 2013 Wiley Periodicals, Inc.",
author = "Olivier Serralbo and Cyril Picard and Christophe Marcelle",
year = "2013",
doi = "10.1002/dvg.22388",
language = "English",
volume = "51",
pages = "372 -- 380",
journal = "Genesis",
issn = "1526-954X",
publisher = "Wiley-Blackwell",
number = "5",

}

Long-term, inducible gene loss-of-function in the chicken embryo. / Serralbo, Olivier; Picard, Cyril; Marcelle, Christophe.

In: Genesis, Vol. 51, No. 5, 2013, p. 372 - 380.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Long-term, inducible gene loss-of-function in the chicken embryo

AU - Serralbo, Olivier

AU - Picard, Cyril

AU - Marcelle, Christophe

PY - 2013

Y1 - 2013

N2 - The use of shRNAmir to down-regulate the expression of genes of interest is a powerful tool for studying gene function during early chick development. However, because of the limitations of electroporation-mediated transgenesis, the down-regulation of genes expressed at late stages of development in specific tissues is difficult to perform. By combining electroporation of a doxycycline-inducible, miR30-based shRNA plasmid with the Tol2 genomic integration system, we are now able to down-regulate the expression of any gene of interest at defined stage of chicken development. genesis 00:1-9. (c) 2013 Wiley Periodicals, Inc.

AB - The use of shRNAmir to down-regulate the expression of genes of interest is a powerful tool for studying gene function during early chick development. However, because of the limitations of electroporation-mediated transgenesis, the down-regulation of genes expressed at late stages of development in specific tissues is difficult to perform. By combining electroporation of a doxycycline-inducible, miR30-based shRNA plasmid with the Tol2 genomic integration system, we are now able to down-regulate the expression of any gene of interest at defined stage of chicken development. genesis 00:1-9. (c) 2013 Wiley Periodicals, Inc.

UR - http://onlinelibrary.wiley.com/doi/10.1002/dvg.22388/pdf

U2 - 10.1002/dvg.22388

DO - 10.1002/dvg.22388

M3 - Article

VL - 51

SP - 372

EP - 380

JO - Genesis

JF - Genesis

SN - 1526-954X

IS - 5

ER -