TY - JOUR
T1 - Long-term administration of tocotrienols and tumor-marker enzyme activities during hepatocarcinogenesis in rats
AU - Rahmat, A.
AU - Ngah, W. Z.W.
AU - Shamaan, N. A.
AU - Gapor, A.
AU - Kadir, K. A.
PY - 1993
Y1 - 1993
N2 - The effects of long-term administration of tocotrienol on hepatocarcinogenesis in rats induced by diethylnitrosamine (DEN) and 2- acetylaminofluorene (AAF) were investigated by determining the activities of γ-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), glutathione S- transferases (GSTs), and glutathione (GSH) levels in blood and liver. Twenty- eight male 7- to 8-wk-old Rattus norwegicus rats, weighing 120-160 g, were used in this study. The rats were divided into four treatment groups: a control group on a basal diet, a group fed a basal diet supplemented with tocotrienol (30 mg/kg food), a group treated with DEN/AAF, and a group treated with DEN/AAF and fed a diet supplemented with tocotrienol (30 mg/kg food). Blood was collected monthly, and GGT, ALP, and GSH levels were determined. The rats were killed after 9 mo, and the livers were examined morphologically. Grayish white nodules (2/liver) were found in all the DEN/AAF-treated rats (n = 10), but only one of the rats treated with DEN/AAF and supplemented with tocotrienol (n = 6) had liver nodules. A significant increase in the level of blood and liver GSH, ALP, and GGT activities was observed in the DEN/AAF-treated rats. Liver GSTs were similarly increased with DEN/AAF treatment. Tocotrienol supplementation attenuated the impact of the carcinogens in the rats.
AB - The effects of long-term administration of tocotrienol on hepatocarcinogenesis in rats induced by diethylnitrosamine (DEN) and 2- acetylaminofluorene (AAF) were investigated by determining the activities of γ-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP), glutathione S- transferases (GSTs), and glutathione (GSH) levels in blood and liver. Twenty- eight male 7- to 8-wk-old Rattus norwegicus rats, weighing 120-160 g, were used in this study. The rats were divided into four treatment groups: a control group on a basal diet, a group fed a basal diet supplemented with tocotrienol (30 mg/kg food), a group treated with DEN/AAF, and a group treated with DEN/AAF and fed a diet supplemented with tocotrienol (30 mg/kg food). Blood was collected monthly, and GGT, ALP, and GSH levels were determined. The rats were killed after 9 mo, and the livers were examined morphologically. Grayish white nodules (2/liver) were found in all the DEN/AAF-treated rats (n = 10), but only one of the rats treated with DEN/AAF and supplemented with tocotrienol (n = 6) had liver nodules. A significant increase in the level of blood and liver GSH, ALP, and GGT activities was observed in the DEN/AAF-treated rats. Liver GSTs were similarly increased with DEN/AAF treatment. Tocotrienol supplementation attenuated the impact of the carcinogens in the rats.
UR - http://www.scopus.com/inward/record.url?scp=0027315199&partnerID=8YFLogxK
M3 - Article
C2 - 8102564
AN - SCOPUS:0027315199
SN - 0899-9007
VL - 9
SP - 229
EP - 232
JO - Nutrition
JF - Nutrition
IS - 3
ER -
