Localization of receptors for calcitonin-gene-related peptide to intraganglionic laminar endings of the mouse esophagus: Peripheral interaction between vagal and spinal afferents?

L Horling, Nigel William Bunnett, Karl Messlinger, Winfried L Neuhuber, Marion Raab

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The calcitonin-gene-related peptide (CGRP) receptor is a heterodimer of calcitonin-receptor-like receptor (CLR) and receptor-activity-modifying protein 1 (RAMP1). Despite the importance of CGRP in regulating gastrointestinal functions, nothing is known about the distribution and function of CLR/RAMP1 in the esophagus, where up to 90 of spinal afferent neurons contain CGRP. We detected CLR/RAMP1 in the mouse esophagus using immunofluorescence and confocal laser scanning microscopy and examined their relationship with neuronal elements of the myenteric plexus. Immunoreactivity for CLR and RAMP1 colocalized with VGLUT2-positive intraganglionic laminar endings (IGLEs), which were contacted by CGRP-positive varicose axons presumably of spinal afferent origin, typically at sites of CRL/RAMP1 immunoreactivity. This provides an anatomical basis for interaction between spinal afferent fibers and IGLEs. Immunoreactive CLR and RAMP1 also colocalized in myenteric neurons. Thus, CGRP-containing spinal afferents may interact with both vagal IGLEs and myenteric neurons in the mouse esophagus, possibly modulating motility reflexes and inflammatory hypersensitivity.
Original languageEnglish
Pages (from-to)321 - 335
Number of pages15
JournalHistochemistry and Cell Biology
Volume141
Issue number3
DOIs
Publication statusPublished - 2014

Cite this

Horling, L ; Bunnett, Nigel William ; Messlinger, Karl ; Neuhuber, Winfried L ; Raab, Marion. / Localization of receptors for calcitonin-gene-related peptide to intraganglionic laminar endings of the mouse esophagus: Peripheral interaction between vagal and spinal afferents?. In: Histochemistry and Cell Biology. 2014 ; Vol. 141, No. 3. pp. 321 - 335.
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title = "Localization of receptors for calcitonin-gene-related peptide to intraganglionic laminar endings of the mouse esophagus: Peripheral interaction between vagal and spinal afferents?",
abstract = "The calcitonin-gene-related peptide (CGRP) receptor is a heterodimer of calcitonin-receptor-like receptor (CLR) and receptor-activity-modifying protein 1 (RAMP1). Despite the importance of CGRP in regulating gastrointestinal functions, nothing is known about the distribution and function of CLR/RAMP1 in the esophagus, where up to 90 of spinal afferent neurons contain CGRP. We detected CLR/RAMP1 in the mouse esophagus using immunofluorescence and confocal laser scanning microscopy and examined their relationship with neuronal elements of the myenteric plexus. Immunoreactivity for CLR and RAMP1 colocalized with VGLUT2-positive intraganglionic laminar endings (IGLEs), which were contacted by CGRP-positive varicose axons presumably of spinal afferent origin, typically at sites of CRL/RAMP1 immunoreactivity. This provides an anatomical basis for interaction between spinal afferent fibers and IGLEs. Immunoreactive CLR and RAMP1 also colocalized in myenteric neurons. Thus, CGRP-containing spinal afferents may interact with both vagal IGLEs and myenteric neurons in the mouse esophagus, possibly modulating motility reflexes and inflammatory hypersensitivity.",
author = "L Horling and Bunnett, {Nigel William} and Karl Messlinger and Neuhuber, {Winfried L} and Marion Raab",
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Localization of receptors for calcitonin-gene-related peptide to intraganglionic laminar endings of the mouse esophagus: Peripheral interaction between vagal and spinal afferents? / Horling, L; Bunnett, Nigel William; Messlinger, Karl; Neuhuber, Winfried L; Raab, Marion.

In: Histochemistry and Cell Biology, Vol. 141, No. 3, 2014, p. 321 - 335.

Research output: Contribution to journalArticleResearchpeer-review

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