Localization of genes encoding metallothionein-like protein (mt2 and smtb) in the brain of zebrafish

Seong Lin Teoh; Satoshi Ogawa; Ishwar S. Parhar

doi:10.1016/j.jchemneu.2015.10.004

Localization of genes encoding metallothionein-like protein (mt2 and smtb) in the brain of zebrafish

Seong Lin Teoh, Satoshi Ogawa, Ishwar S. Parhar

Research output: Contribution to journal › Article › Research › peer-review

4 Citations (Scopus)

Abstract

Metallothionein (MT) is a small cysteine-rich heavy metal-binding protein involved in metal homeostasis, detoxification and free radical-scavenging. MT is ubiquitously expressed in several tissues, but its role in the central nervous system is not well understood. In this study, we identified two MT homologous genes (. mt2 and smtb) in the zebrafish. Digoxigenin-. in situ hybridization showed the expression of mt2 and smtb genes in the ventricular layers in the telencephalon, diencephalon, mesencephalon and rhombencephalon, most of which are cell proliferating regions in the brain of zebrafish. Cellular characteristics of MT genes expressing cells were examined by double-labelling with markers for neurons (HuC/D) and astrocytes (glial fibrillary acidic protein, GFAP and S100 protein) and cell proliferation marker (PCNA). mt2 and smtb mRNAs are expressed in neurons and not in astrocytes, and they were co-localized with PCNA. These results suggest that mt2 and smtb may play an important role in neurogenesis and neuroprotection.

Original language	English
Pages (from-to)	20-32
Number of pages	13
Journal	Journal of Chemical Neuroanatomy
Volume	70
DOIs	https://doi.org/10.1016/j.jchemneu.2015.10.004
Publication status	Published - Dec 2015

Keywords

GFAP
HuC/D
Neurogenesis
Neuroprotection
Teleost

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10.1016/j.jchemneu.2015.10.004

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Cite this

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title = "Localization of genes encoding metallothionein-like protein (mt2 and smtb) in the brain of zebrafish",

abstract = "Metallothionein (MT) is a small cysteine-rich heavy metal-binding protein involved in metal homeostasis, detoxification and free radical-scavenging. MT is ubiquitously expressed in several tissues, but its role in the central nervous system is not well understood. In this study, we identified two MT homologous genes (. mt2 and smtb) in the zebrafish. Digoxigenin-. in situ hybridization showed the expression of mt2 and smtb genes in the ventricular layers in the telencephalon, diencephalon, mesencephalon and rhombencephalon, most of which are cell proliferating regions in the brain of zebrafish. Cellular characteristics of MT genes expressing cells were examined by double-labelling with markers for neurons (HuC/D) and astrocytes (glial fibrillary acidic protein, GFAP and S100 protein) and cell proliferation marker (PCNA). mt2 and smtb mRNAs are expressed in neurons and not in astrocytes, and they were co-localized with PCNA. These results suggest that mt2 and smtb may play an important role in neurogenesis and neuroprotection.",

keywords = "GFAP, HuC/D, Neurogenesis, Neuroprotection, Teleost",

author = "Teoh, {Seong Lin} and Satoshi Ogawa and Parhar, {Ishwar S.}",

note = "Funding Information: We thank Ms. Mageswary Sivalingam, Ms. Ng Kai We, and Ms. Rachel Shalini, for their technical advice. We also thank The National University of Malaysia for the Higher Degree Research Scholarship to T.S.L. This work was supported by the Malaysian Ministry of Science, Technology and Innovation ; Grant numbers: 02-02-10-SF0044 and 02-02-10-SF0161 . Publisher Copyright: {\textcopyright} 2015 Elsevier B.V. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.",

year = "2015",

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doi = "10.1016/j.jchemneu.2015.10.004",

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AU - Teoh, Seong Lin

AU - Ogawa, Satoshi

AU - Parhar, Ishwar S.

N1 - Funding Information: We thank Ms. Mageswary Sivalingam, Ms. Ng Kai We, and Ms. Rachel Shalini, for their technical advice. We also thank The National University of Malaysia for the Higher Degree Research Scholarship to T.S.L. This work was supported by the Malaysian Ministry of Science, Technology and Innovation ; Grant numbers: 02-02-10-SF0044 and 02-02-10-SF0161 . Publisher Copyright: © 2015 Elsevier B.V. Copyright: Copyright 2016 Elsevier B.V., All rights reserved.

PY - 2015/12

Y1 - 2015/12

N2 - Metallothionein (MT) is a small cysteine-rich heavy metal-binding protein involved in metal homeostasis, detoxification and free radical-scavenging. MT is ubiquitously expressed in several tissues, but its role in the central nervous system is not well understood. In this study, we identified two MT homologous genes (. mt2 and smtb) in the zebrafish. Digoxigenin-. in situ hybridization showed the expression of mt2 and smtb genes in the ventricular layers in the telencephalon, diencephalon, mesencephalon and rhombencephalon, most of which are cell proliferating regions in the brain of zebrafish. Cellular characteristics of MT genes expressing cells were examined by double-labelling with markers for neurons (HuC/D) and astrocytes (glial fibrillary acidic protein, GFAP and S100 protein) and cell proliferation marker (PCNA). mt2 and smtb mRNAs are expressed in neurons and not in astrocytes, and they were co-localized with PCNA. These results suggest that mt2 and smtb may play an important role in neurogenesis and neuroprotection.

AB - Metallothionein (MT) is a small cysteine-rich heavy metal-binding protein involved in metal homeostasis, detoxification and free radical-scavenging. MT is ubiquitously expressed in several tissues, but its role in the central nervous system is not well understood. In this study, we identified two MT homologous genes (. mt2 and smtb) in the zebrafish. Digoxigenin-. in situ hybridization showed the expression of mt2 and smtb genes in the ventricular layers in the telencephalon, diencephalon, mesencephalon and rhombencephalon, most of which are cell proliferating regions in the brain of zebrafish. Cellular characteristics of MT genes expressing cells were examined by double-labelling with markers for neurons (HuC/D) and astrocytes (glial fibrillary acidic protein, GFAP and S100 protein) and cell proliferation marker (PCNA). mt2 and smtb mRNAs are expressed in neurons and not in astrocytes, and they were co-localized with PCNA. These results suggest that mt2 and smtb may play an important role in neurogenesis and neuroprotection.

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