TY - JOUR
T1 - Localization of an immunoinhibitory epitope of the cysteine proteinase, cathepsin l
AU - Coetzer, Theresa H.T.
AU - Pike, Robert N.
AU - Dennison, Clive
PY - 1992/1/1
Y1 - 1992/1/1
N2 - Antibodies, raised in chickens (IgY) and rabbits (IgG) against the lysosomal proteinase cathepsin L, targeted the enzyme in an ELISA and Western blot. In contrast to the rabbit IgG, the chicken IgY was immunoinhibitory towards cathepsin L. An epitope that elicits immunoinhibitory antibodies has been localized to an active site-associated peptide sequence. The corresponding free peptide, coated down in an ELISA, is recognised by the chicken IgY, but not the rabbit IgG. This peptide was able to inhibit the immunoinhibition of cathepsin L by chicken anti-cathepsin L IgY, suggesting its complete or partial identity with an immunogenic epitope for chickens in whole cathepsin L.
AB - Antibodies, raised in chickens (IgY) and rabbits (IgG) against the lysosomal proteinase cathepsin L, targeted the enzyme in an ELISA and Western blot. In contrast to the rabbit IgG, the chicken IgY was immunoinhibitory towards cathepsin L. An epitope that elicits immunoinhibitory antibodies has been localized to an active site-associated peptide sequence. The corresponding free peptide, coated down in an ELISA, is recognised by the chicken IgY, but not the rabbit IgG. This peptide was able to inhibit the immunoinhibition of cathepsin L by chicken anti-cathepsin L IgY, suggesting its complete or partial identity with an immunogenic epitope for chickens in whole cathepsin L.
UR - http://www.scopus.com/inward/record.url?scp=0026782007&partnerID=8YFLogxK
U2 - 10.3109/08820139209069386
DO - 10.3109/08820139209069386
M3 - Article
C2 - 1385322
AN - SCOPUS:0026782007
SN - 0882-0139
VL - 21
SP - 495
EP - 506
JO - Immunological Investigations
JF - Immunological Investigations
IS - 6
ER -