Local Modulation of Antigen-Presenting Cell Development after Resolution of Pneumonia Induces Long-Term Susceptibility to Secondary Infections

Antoine Roquilly, Hamish E.G. McWilliam, Cedric Jacqueline, Zehua Tian, Raphael Cinotti, Marie Rimbert, Linda Wakim, Irina Caminschi, Mireille H. Lahoud, Gabrielle T. Belz, Axel Kallies, Justine D. Mintern, Karim Asehnoune, Jose A. Villadangos

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33 Citations (Scopus)

Abstract

Lung infections cause prolonged immune alterations and elevated susceptibility to secondary pneumonia. We found that, after resolution of primary viral or bacterial pneumonia, dendritic cells (DC), and macrophages exhibited poor antigen-presentation capacity and secretion of immunogenic cytokines. Development of these “paralyzed” DCs and macrophages depended on the immunosuppressive microenvironment established upon resolution of primary infection, which involved regulatory T (Treg) cells and the cytokine TGF-β. Paralyzed DCs secreted TGF-β and induced local Treg cell accumulation. They also expressed lower amounts of IRF4, a transcription factor associated with increased antigen-presentation capacity, and higher amounts of Blimp1, a transcription factor associated with tolerogenic functions, than DCs present during primary infection. Blimp1 expression in DC of humans suffering sepsis or trauma correlated with severity and complicated outcomes. Our findings describe mechanisms underlying sepsis- and trauma-induced immunosuppression, reveal prognostic markers of susceptibility to secondary infections and identify potential targets for therapeutic intervention. Following a severe primary infection, the risk of developing pneumonia increases due to acquired immune defects collectively known as sepsis-induced immunosuppression. Roquilly et al. show that resolution of the primary infection changed the local environment, leading to the development of DCs and macrophages that are functionally impaired in terms of T cell activation, and instead exhibit tolerogenic properties that contribute to immune suppression.

Original languageEnglish
Pages (from-to)135-147.e5
Number of pages17
JournalImmunity
Volume47
Issue number1
DOIs
Publication statusPublished - 18 Jul 2017

Keywords

  • Bacterial Infection
  • Dendritic Cells
  • Influenza Virus
  • interleukin 12
  • mucosal immunology
  • Natural Killer Cells
  • pneumonia
  • Transforming Growth Factor Beta
  • Trauma
  • Treg cells

Cite this

Roquilly, A., McWilliam, H. E. G., Jacqueline, C., Tian, Z., Cinotti, R., Rimbert, M., ... Villadangos, J. A. (2017). Local Modulation of Antigen-Presenting Cell Development after Resolution of Pneumonia Induces Long-Term Susceptibility to Secondary Infections. Immunity, 47(1), 135-147.e5. https://doi.org/10.1016/j.immuni.2017.06.021
Roquilly, Antoine ; McWilliam, Hamish E.G. ; Jacqueline, Cedric ; Tian, Zehua ; Cinotti, Raphael ; Rimbert, Marie ; Wakim, Linda ; Caminschi, Irina ; Lahoud, Mireille H. ; Belz, Gabrielle T. ; Kallies, Axel ; Mintern, Justine D. ; Asehnoune, Karim ; Villadangos, Jose A. / Local Modulation of Antigen-Presenting Cell Development after Resolution of Pneumonia Induces Long-Term Susceptibility to Secondary Infections. In: Immunity. 2017 ; Vol. 47, No. 1. pp. 135-147.e5.
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title = "Local Modulation of Antigen-Presenting Cell Development after Resolution of Pneumonia Induces Long-Term Susceptibility to Secondary Infections",
abstract = "Lung infections cause prolonged immune alterations and elevated susceptibility to secondary pneumonia. We found that, after resolution of primary viral or bacterial pneumonia, dendritic cells (DC), and macrophages exhibited poor antigen-presentation capacity and secretion of immunogenic cytokines. Development of these “paralyzed” DCs and macrophages depended on the immunosuppressive microenvironment established upon resolution of primary infection, which involved regulatory T (Treg) cells and the cytokine TGF-β. Paralyzed DCs secreted TGF-β and induced local Treg cell accumulation. They also expressed lower amounts of IRF4, a transcription factor associated with increased antigen-presentation capacity, and higher amounts of Blimp1, a transcription factor associated with tolerogenic functions, than DCs present during primary infection. Blimp1 expression in DC of humans suffering sepsis or trauma correlated with severity and complicated outcomes. Our findings describe mechanisms underlying sepsis- and trauma-induced immunosuppression, reveal prognostic markers of susceptibility to secondary infections and identify potential targets for therapeutic intervention. Following a severe primary infection, the risk of developing pneumonia increases due to acquired immune defects collectively known as sepsis-induced immunosuppression. Roquilly et al. show that resolution of the primary infection changed the local environment, leading to the development of DCs and macrophages that are functionally impaired in terms of T cell activation, and instead exhibit tolerogenic properties that contribute to immune suppression.",
keywords = "Bacterial Infection, Dendritic Cells, Influenza Virus, interleukin 12, mucosal immunology, Natural Killer Cells, pneumonia, Transforming Growth Factor Beta, Trauma, Treg cells",
author = "Antoine Roquilly and McWilliam, {Hamish E.G.} and Cedric Jacqueline and Zehua Tian and Raphael Cinotti and Marie Rimbert and Linda Wakim and Irina Caminschi and Lahoud, {Mireille H.} and Belz, {Gabrielle T.} and Axel Kallies and Mintern, {Justine D.} and Karim Asehnoune and Villadangos, {Jose A.}",
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Roquilly, A, McWilliam, HEG, Jacqueline, C, Tian, Z, Cinotti, R, Rimbert, M, Wakim, L, Caminschi, I, Lahoud, MH, Belz, GT, Kallies, A, Mintern, JD, Asehnoune, K & Villadangos, JA 2017, 'Local Modulation of Antigen-Presenting Cell Development after Resolution of Pneumonia Induces Long-Term Susceptibility to Secondary Infections', Immunity, vol. 47, no. 1, pp. 135-147.e5. https://doi.org/10.1016/j.immuni.2017.06.021

Local Modulation of Antigen-Presenting Cell Development after Resolution of Pneumonia Induces Long-Term Susceptibility to Secondary Infections. / Roquilly, Antoine; McWilliam, Hamish E.G.; Jacqueline, Cedric; Tian, Zehua; Cinotti, Raphael; Rimbert, Marie; Wakim, Linda; Caminschi, Irina; Lahoud, Mireille H.; Belz, Gabrielle T.; Kallies, Axel; Mintern, Justine D.; Asehnoune, Karim; Villadangos, Jose A.

In: Immunity, Vol. 47, No. 1, 18.07.2017, p. 135-147.e5.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Local Modulation of Antigen-Presenting Cell Development after Resolution of Pneumonia Induces Long-Term Susceptibility to Secondary Infections

AU - Roquilly, Antoine

AU - McWilliam, Hamish E.G.

AU - Jacqueline, Cedric

AU - Tian, Zehua

AU - Cinotti, Raphael

AU - Rimbert, Marie

AU - Wakim, Linda

AU - Caminschi, Irina

AU - Lahoud, Mireille H.

AU - Belz, Gabrielle T.

AU - Kallies, Axel

AU - Mintern, Justine D.

AU - Asehnoune, Karim

AU - Villadangos, Jose A.

PY - 2017/7/18

Y1 - 2017/7/18

N2 - Lung infections cause prolonged immune alterations and elevated susceptibility to secondary pneumonia. We found that, after resolution of primary viral or bacterial pneumonia, dendritic cells (DC), and macrophages exhibited poor antigen-presentation capacity and secretion of immunogenic cytokines. Development of these “paralyzed” DCs and macrophages depended on the immunosuppressive microenvironment established upon resolution of primary infection, which involved regulatory T (Treg) cells and the cytokine TGF-β. Paralyzed DCs secreted TGF-β and induced local Treg cell accumulation. They also expressed lower amounts of IRF4, a transcription factor associated with increased antigen-presentation capacity, and higher amounts of Blimp1, a transcription factor associated with tolerogenic functions, than DCs present during primary infection. Blimp1 expression in DC of humans suffering sepsis or trauma correlated with severity and complicated outcomes. Our findings describe mechanisms underlying sepsis- and trauma-induced immunosuppression, reveal prognostic markers of susceptibility to secondary infections and identify potential targets for therapeutic intervention. Following a severe primary infection, the risk of developing pneumonia increases due to acquired immune defects collectively known as sepsis-induced immunosuppression. Roquilly et al. show that resolution of the primary infection changed the local environment, leading to the development of DCs and macrophages that are functionally impaired in terms of T cell activation, and instead exhibit tolerogenic properties that contribute to immune suppression.

AB - Lung infections cause prolonged immune alterations and elevated susceptibility to secondary pneumonia. We found that, after resolution of primary viral or bacterial pneumonia, dendritic cells (DC), and macrophages exhibited poor antigen-presentation capacity and secretion of immunogenic cytokines. Development of these “paralyzed” DCs and macrophages depended on the immunosuppressive microenvironment established upon resolution of primary infection, which involved regulatory T (Treg) cells and the cytokine TGF-β. Paralyzed DCs secreted TGF-β and induced local Treg cell accumulation. They also expressed lower amounts of IRF4, a transcription factor associated with increased antigen-presentation capacity, and higher amounts of Blimp1, a transcription factor associated with tolerogenic functions, than DCs present during primary infection. Blimp1 expression in DC of humans suffering sepsis or trauma correlated with severity and complicated outcomes. Our findings describe mechanisms underlying sepsis- and trauma-induced immunosuppression, reveal prognostic markers of susceptibility to secondary infections and identify potential targets for therapeutic intervention. Following a severe primary infection, the risk of developing pneumonia increases due to acquired immune defects collectively known as sepsis-induced immunosuppression. Roquilly et al. show that resolution of the primary infection changed the local environment, leading to the development of DCs and macrophages that are functionally impaired in terms of T cell activation, and instead exhibit tolerogenic properties that contribute to immune suppression.

KW - Bacterial Infection

KW - Dendritic Cells

KW - Influenza Virus

KW - interleukin 12

KW - mucosal immunology

KW - Natural Killer Cells

KW - pneumonia

KW - Transforming Growth Factor Beta

KW - Trauma

KW - Treg cells

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DO - 10.1016/j.immuni.2017.06.021

M3 - Article

VL - 47

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