TY - JOUR
T1 - Loading the dice
T2 - The orientation of virus-like particles adsorbed on titanate assisted organosilanized surfaces
AU - Moreno-Cerrada, Daniel
AU - Rodríguez, Chloe
AU - Moreno-Madrid, Francisco
AU - Selivanovitch, Ekaterina
AU - Douglas, Trevor
AU - De Pablo, Pedro J.
AU - Manso Silván, Miguel
N1 - Funding Information:
The authors thank L. García Pelayo for his technical assistance during materials processing. They acknowledge MSC funding provided by the European Commission through FP7 grant THINFACE (No. ITN GA 607232) and by Ministerio de Economía and Competitividad of Spain grant Bio2DSense (No. CTQ2017-84309-C2-2-R). P.J.P. acknowledges FIS2017-89549-R and the “María de Maeztu” Program for Units of Excellence in R&D (No. MDM-2014-0377). P.J.P. and T.D. acknowledge the Human Frontiers Science Program (No. RGP0012/2018).
Publisher Copyright:
© 2019 Author(s).
PY - 2019/1
Y1 - 2019/1
N2 - The organization of virus-like particles (VLPs) on surfaces is a relevant matter for both fundamental and biomedical sciences. In this work, the authors have tailored surfaces with different surface tension components aiming at finding a relationship with the affinity of the different geometric/surface features of icosahedral P22 VLPs. The surfaces have been prepared by titanate assisted organosilanization with glycidyloxy, amino, and perfluoro silanes. Vibrational and photoelectron spectroscopies have allowed identifying the different functional groups of the organosilanes on the surfaces. Atomic force microscopy (AFM) showed that, irrespective of the organosilane used, the final root mean square roughness remains below 1 nm. Contact angle analyses confirm the effective formation of a set of surface chemistries exhibiting different balance among surface tension components. The study of the adsorption of P22 VLPs has involved the analysis of the dynamics of virus immobilization by fluorescence microscopy and the interpretation of the final VLP orientation by AFM. These analyses give rise to statistical distributions pointing to a higher affinity of VLPs toward perfluorinated surfaces, with a dominant fivefold conformation on this hydrophobic surface, but threefold and twofold symmetries dominating on hydrophilic surfaces. These results can be explained in terms of a reinforced hydrophobic interaction between the perfluorinated surface and the dominating hydrophobic residues present at the P22 pentons.
AB - The organization of virus-like particles (VLPs) on surfaces is a relevant matter for both fundamental and biomedical sciences. In this work, the authors have tailored surfaces with different surface tension components aiming at finding a relationship with the affinity of the different geometric/surface features of icosahedral P22 VLPs. The surfaces have been prepared by titanate assisted organosilanization with glycidyloxy, amino, and perfluoro silanes. Vibrational and photoelectron spectroscopies have allowed identifying the different functional groups of the organosilanes on the surfaces. Atomic force microscopy (AFM) showed that, irrespective of the organosilane used, the final root mean square roughness remains below 1 nm. Contact angle analyses confirm the effective formation of a set of surface chemistries exhibiting different balance among surface tension components. The study of the adsorption of P22 VLPs has involved the analysis of the dynamics of virus immobilization by fluorescence microscopy and the interpretation of the final VLP orientation by AFM. These analyses give rise to statistical distributions pointing to a higher affinity of VLPs toward perfluorinated surfaces, with a dominant fivefold conformation on this hydrophobic surface, but threefold and twofold symmetries dominating on hydrophilic surfaces. These results can be explained in terms of a reinforced hydrophobic interaction between the perfluorinated surface and the dominating hydrophobic residues present at the P22 pentons.
UR - http://www.scopus.com/inward/record.url?scp=85060633633&partnerID=8YFLogxK
U2 - 10.1116/1.5077010
DO - 10.1116/1.5077010
M3 - Article
C2 - 30691269
AN - SCOPUS:85060633633
SN - 1559-4106
VL - 14
JO - Biointerphases
JF - Biointerphases
IS - 1
M1 - 011001
ER -