Live cell imaging and profiling of cysteine cathepsin activity using a quenched activity-based probe

Laura E. Edgington-Mitchell, Matthew Bogyo, Martijn Verdoes

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

Abstract

Since protease activity is highly regulated by structural and environmental influences, the abundance of a protease often does not directly correlate with its activity. Because in most of the cases it is the activity of a protease that gives rise to its biological relevance, tools to report on this activity are of great value to the research community. Activity-based probes (ABPs) are small molecule tools that allow for the monitoring and profiling of protease activities in complex biological systems. The class of fluorescent quenched ABPs (qABPs), being intrinsically “dark” and only emitting fluorescence after reaction with the target protease, are ideally suited for imaging techniques such as small animal noninvasive fluorescence imaging and live cell fl uorescence microscopy. An additional powerful characteristic of qABPs is their covalent and irreversible modification of the labeled protease, enabling in-depth target characterization. Here we describe the synthesis of a pan-cysteine cathepsin qABP BMV109 and the application of this probe to live cell fl uorescence imaging and fluorescent SDS-PAGE cysteine cathepsin activity profiling.

Original languageEnglish
Title of host publicationActivity-Based Proteomics
Subtitle of host publicationMethods and Protocols
EditorsHerman S. Overkleeft, Bogdan I. Florea
Place of PublicationNew York NY USA
PublisherSpringer
Pages145-159
Number of pages15
ISBN (Electronic)9781493964390
ISBN (Print)9781493964376
DOIs
Publication statusPublished - 25 Oct 2017

Publication series

NameMethods in Molecular Biology
PublisherSpringer
Volume1491
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Activity-based probe
  • Cysteine cathepsin
  • Fluorescent SDS-PAGE
  • Live cell imaging
  • Protease

Cite this

Edgington-Mitchell, L. E., Bogyo, M., & Verdoes, M. (2017). Live cell imaging and profiling of cysteine cathepsin activity using a quenched activity-based probe. In H. S. Overkleeft, & B. I. Florea (Eds.), Activity-Based Proteomics: Methods and Protocols (pp. 145-159). (Methods in Molecular Biology; Vol. 1491). New York NY USA: Springer. https://doi.org/10.1007/978-1-4939-6439-0_11
Edgington-Mitchell, Laura E. ; Bogyo, Matthew ; Verdoes, Martijn. / Live cell imaging and profiling of cysteine cathepsin activity using a quenched activity-based probe. Activity-Based Proteomics: Methods and Protocols. editor / Herman S. Overkleeft ; Bogdan I. Florea. New York NY USA : Springer, 2017. pp. 145-159 (Methods in Molecular Biology).
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Edgington-Mitchell, LE, Bogyo, M & Verdoes, M 2017, Live cell imaging and profiling of cysteine cathepsin activity using a quenched activity-based probe. in HS Overkleeft & BI Florea (eds), Activity-Based Proteomics: Methods and Protocols. Methods in Molecular Biology, vol. 1491, Springer, New York NY USA, pp. 145-159. https://doi.org/10.1007/978-1-4939-6439-0_11

Live cell imaging and profiling of cysteine cathepsin activity using a quenched activity-based probe. / Edgington-Mitchell, Laura E.; Bogyo, Matthew; Verdoes, Martijn.

Activity-Based Proteomics: Methods and Protocols. ed. / Herman S. Overkleeft; Bogdan I. Florea. New York NY USA : Springer, 2017. p. 145-159 (Methods in Molecular Biology; Vol. 1491).

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Otherpeer-review

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Edgington-Mitchell LE, Bogyo M, Verdoes M. Live cell imaging and profiling of cysteine cathepsin activity using a quenched activity-based probe. In Overkleeft HS, Florea BI, editors, Activity-Based Proteomics: Methods and Protocols. New York NY USA: Springer. 2017. p. 145-159. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6439-0_11