'Live and Large': Super-Resolution Optical Fluctuation Imaging (SOFI) and Expansion Microscopy (ExM) of Microtubule Remodelling by Rabies Virus P Protein

Ashley M. Rozario, Fabian Zwettler, Sam Duwé, Riley Hargreaves, Aaron Brice, Peter Dedecker, Markus Sauer, Gregory W. Moseley, Donna R. Whelan, Toby D.M. Bell

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The field of super-resolution microscopy continues to progress rapidly, both in terms of evolving techniques and methodologies as well as in the development of new multi-disciplinary applications. Two current drivers of innovation are increasing the possible resolution gain and application in live samples. Super-resolution optical fluctuation imaging (SOFI) is well suited to live samples while expansion microscopy (ExM) enables obtainment of sub-diffraction information via conventional imaging. In this Highlight we provide a brief outline of these methods and report results from application of SOFI and ExM in our on-going study into microtubule remodelling by rabies virus P proteins. We show that MT bundles in live cells transfected with rabies virus P3 protein can be visualised using SOFI in a time-lapse fashion for up to half an hour and can be expanded using current Pro-ExM protocols and imaged using conventional microscopy.

Original languageEnglish
Article numberCH19571
Number of pages7
JournalAustralian Journal of Chemistry
DOIs
Publication statusAccepted/In press - 27 Apr 2020

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