Liquid marble as microbioreactor for bioengineering applications

Fatemeh Sarvi; Kanika Jain; Layla Alhasan; Tina Arbatan; Wei Shen; Peggy P.Y. Chan

doi:10.1117/12.2201110

Liquid marble as microbioreactor for bioengineering applications

Fatemeh Sarvi
, Kanika Jain
, Layla Alhasan
, Tina Arbatan
, Wei Shen
, Peggy P.Y. Chan

Chemical & Biological Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

Abstract

This paper reports the use of liquid marbles (LMs) as miniature bioreactors to produce three-dimensional (3D) spheroids including tumor-like spheriods from cancer cells and embryoid bodies (EBs) from stem cells. A liquid marble microbioreactor is prepared by placing a drop of cell suspension onto a polytetrafluoroethylene (PTFE) particle bed. Without the addition of growth factors, suspended EBs from liquid marbles exhibit spontaneous contraction. These results indicate that the liquid marble provides a suitable microenvironment to induce EB formation and spontaneous cardiac differentiation. The EBs were further plated onto gelatin-coated tissue culture dishes. Plated EBs express mature cardiomyocyte marker cardiac troponinT (cTnT), indicating that these EBs have differentiated into functional cardiomyocytes. The cardiomyocytes generated using this liquid marble approach could be useful for transplantation.

Original language	English
Title of host publication	Micro+Nano Materials, Devices, and Systems
Subtitle of host publication	Sydney NSW Australia; 6-9 December 2015
Editors	Benjamin J. Eggleton, Stefano Palomba
Place of Publication	Bellingham WA USA
Publisher	SPIE - International Society for Optical Engineering
Number of pages	6
ISBN (Electronic)	9781628418903
DOIs	https://doi.org/10.1117/12.2201110
Publication status	Published - 2015
Event	SPIE Micro+Nano Materials, Devices, and Applications Symposium 2015 - The University of Sydney, Sydney, Australia Duration: 6 Dec 2015 → 9 Dec 2015 http://spie.org/conferences-and-exhibitions/micronano-materials-devices-and-applications?SSO=1

Publication series

Name	Proceedings of SPIE
Publisher	The International Society for Optical Engineering
Volume	9668
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	SPIE Micro+Nano Materials, Devices, and Applications Symposium 2015
Country/Territory	Australia
City	Sydney
Period	6/12/15 → 9/12/15
Internet address	http://spie.org/conferences-and-exhibitions/micronano-materials-devices-and-applications?SSO=1

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

cardiogenesis
embryoid bodies
liquid marbles
Micro-bioreactor
spheroids
stem cell culture
stem cell differentiation

Access to Document

10.1117/12.2201110Licence: Unspecified

Cite this

Sarvi, F., Jain, K., Alhasan, L., Arbatan, T., Shen, W., & Chan, P. P. Y. (2015). Liquid marble as microbioreactor for bioengineering applications. In B. J. Eggleton, & S. Palomba (Eds.), Micro+Nano Materials, Devices, and Systems: Sydney NSW Australia; 6-9 December 2015 Article 966852 (Proceedings of SPIE; Vol. 9668). SPIE - International Society for Optical Engineering. https://doi.org/10.1117/12.2201110

@inproceedings{5888d80a1d8d443bb94e7bf6b746f957,

title = "Liquid marble as microbioreactor for bioengineering applications",

abstract = "This paper reports the use of liquid marbles (LMs) as miniature bioreactors to produce three-dimensional (3D) spheroids including tumor-like spheriods from cancer cells and embryoid bodies (EBs) from stem cells. A liquid marble microbioreactor is prepared by placing a drop of cell suspension onto a polytetrafluoroethylene (PTFE) particle bed. Without the addition of growth factors, suspended EBs from liquid marbles exhibit spontaneous contraction. These results indicate that the liquid marble provides a suitable microenvironment to induce EB formation and spontaneous cardiac differentiation. The EBs were further plated onto gelatin-coated tissue culture dishes. Plated EBs express mature cardiomyocyte marker cardiac troponinT (cTnT), indicating that these EBs have differentiated into functional cardiomyocytes. The cardiomyocytes generated using this liquid marble approach could be useful for transplantation.",

keywords = "cardiogenesis, embryoid bodies, liquid marbles, Micro-bioreactor, spheroids, stem cell culture, stem cell differentiation",

author = "Fatemeh Sarvi and Kanika Jain and Layla Alhasan and Tina Arbatan and Wei Shen and Chan, \{Peggy P.Y.\}",

year = "2015",

doi = "10.1117/12.2201110",

language = "English",

series = "Proceedings of SPIE",

publisher = "SPIE - International Society for Optical Engineering",

editor = "Eggleton, \{Benjamin J.\} and Stefano Palomba",

booktitle = "Micro+Nano Materials, Devices, and Systems",

address = "United States of America",

note = "SPIE Micro+Nano Materials, Devices, and Applications Symposium 2015 ; Conference date: 06-12-2015 Through 09-12-2015",

url = "http://spie.org/conferences-and-exhibitions/micronano-materials-devices-and-applications?SSO=1",

}

Sarvi, F, Jain, K, Alhasan, L, Arbatan, T, Shen, W & Chan, PPY 2015, Liquid marble as microbioreactor for bioengineering applications. in BJ Eggleton & S Palomba (eds), Micro+Nano Materials, Devices, and Systems: Sydney NSW Australia; 6-9 December 2015., 966852, Proceedings of SPIE, vol. 9668, SPIE - International Society for Optical Engineering, Bellingham WA USA, SPIE Micro+Nano Materials, Devices, and Applications Symposium 2015, Sydney, New South Wales, Australia, 6/12/15. https://doi.org/10.1117/12.2201110

Liquid marble as microbioreactor for bioengineering applications. / Sarvi, Fatemeh; Jain, Kanika; Alhasan, Layla et al.
Micro+Nano Materials, Devices, and Systems: Sydney NSW Australia; 6-9 December 2015. ed. / Benjamin J. Eggleton; Stefano Palomba. Bellingham WA USA: SPIE - International Society for Optical Engineering, 2015. 966852 (Proceedings of SPIE; Vol. 9668).

Research output: Chapter in Book/Report/Conference proceeding › Conference Paper › Research › peer-review

TY - GEN

T1 - Liquid marble as microbioreactor for bioengineering applications

AU - Sarvi, Fatemeh

AU - Jain, Kanika

AU - Alhasan, Layla

AU - Arbatan, Tina

AU - Shen, Wei

AU - Chan, Peggy P.Y.

PY - 2015

Y1 - 2015

N2 - This paper reports the use of liquid marbles (LMs) as miniature bioreactors to produce three-dimensional (3D) spheroids including tumor-like spheriods from cancer cells and embryoid bodies (EBs) from stem cells. A liquid marble microbioreactor is prepared by placing a drop of cell suspension onto a polytetrafluoroethylene (PTFE) particle bed. Without the addition of growth factors, suspended EBs from liquid marbles exhibit spontaneous contraction. These results indicate that the liquid marble provides a suitable microenvironment to induce EB formation and spontaneous cardiac differentiation. The EBs were further plated onto gelatin-coated tissue culture dishes. Plated EBs express mature cardiomyocyte marker cardiac troponinT (cTnT), indicating that these EBs have differentiated into functional cardiomyocytes. The cardiomyocytes generated using this liquid marble approach could be useful for transplantation.

AB - This paper reports the use of liquid marbles (LMs) as miniature bioreactors to produce three-dimensional (3D) spheroids including tumor-like spheriods from cancer cells and embryoid bodies (EBs) from stem cells. A liquid marble microbioreactor is prepared by placing a drop of cell suspension onto a polytetrafluoroethylene (PTFE) particle bed. Without the addition of growth factors, suspended EBs from liquid marbles exhibit spontaneous contraction. These results indicate that the liquid marble provides a suitable microenvironment to induce EB formation and spontaneous cardiac differentiation. The EBs were further plated onto gelatin-coated tissue culture dishes. Plated EBs express mature cardiomyocyte marker cardiac troponinT (cTnT), indicating that these EBs have differentiated into functional cardiomyocytes. The cardiomyocytes generated using this liquid marble approach could be useful for transplantation.

KW - cardiogenesis

KW - embryoid bodies

KW - liquid marbles

KW - Micro-bioreactor

KW - spheroids

KW - stem cell culture

KW - stem cell differentiation

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U2 - 10.1117/12.2201110

DO - 10.1117/12.2201110

M3 - Conference Paper

AN - SCOPUS:84959907790

T3 - Proceedings of SPIE

BT - Micro+Nano Materials, Devices, and Systems

A2 - Eggleton, Benjamin J.

A2 - Palomba, Stefano

PB - SPIE - International Society for Optical Engineering

CY - Bellingham WA USA

T2 - SPIE Micro+Nano Materials, Devices, and Applications Symposium 2015

Y2 - 6 December 2015 through 9 December 2015

ER -

Sarvi F, Jain K, Alhasan L, Arbatan T, Shen W, Chan PPY. Liquid marble as microbioreactor for bioengineering applications. In Eggleton BJ, Palomba S, editors, Micro+Nano Materials, Devices, and Systems: Sydney NSW Australia; 6-9 December 2015. Bellingham WA USA: SPIE - International Society for Optical Engineering. 2015. 966852. (Proceedings of SPIE). doi: 10.1117/12.2201110

Liquid marble as microbioreactor for bioengineering applications

Abstract

Publication series

Conference

UN SDGs

Keywords

Access to Document

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