Liquid marble as microbioreactor for bioengineering applications

Fatemeh Sarvi, Kanika Jain, Layla Alhasan, Tina Arbatan, Wei Shen, Peggy P.Y. Chan

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review


This paper reports the use of liquid marbles (LMs) as miniature bioreactors to produce three-dimensional (3D) spheroids including tumor-like spheriods from cancer cells and embryoid bodies (EBs) from stem cells. A liquid marble microbioreactor is prepared by placing a drop of cell suspension onto a polytetrafluoroethylene (PTFE) particle bed. Without the addition of growth factors, suspended EBs from liquid marbles exhibit spontaneous contraction. These results indicate that the liquid marble provides a suitable microenvironment to induce EB formation and spontaneous cardiac differentiation. The EBs were further plated onto gelatin-coated tissue culture dishes. Plated EBs express mature cardiomyocyte marker cardiac troponinT (cTnT), indicating that these EBs have differentiated into functional cardiomyocytes. The cardiomyocytes generated using this liquid marble approach could be useful for transplantation.

Original languageEnglish
Title of host publicationMicro+Nano Materials, Devices, and Systems
Subtitle of host publicationSydney NSW Australia; 6-9 December 2015
EditorsBenjamin J. Eggleton, Stefano Palomba
Place of PublicationBellingham WA USA
Number of pages6
ISBN (Electronic)9781628418903
Publication statusPublished - 2015
EventSPIE Micro+Nano Materials, Devices, and Applications Symposium 2015 - The University of Sydney, Sydney, Australia
Duration: 6 Dec 20159 Dec 2015

Publication series

NameProceedings of SPIE
PublisherThe International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceSPIE Micro+Nano Materials, Devices, and Applications Symposium 2015
Internet address


  • cardiogenesis
  • embryoid bodies
  • liquid marbles
  • Micro-bioreactor
  • spheroids
  • stem cell culture
  • stem cell differentiation

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