Liquid crystalline systems of phytantriol and glyceryl monooleate containing a hydrophilic protein: characterisation, swelling and release kinetics

S B Rizwan, Tracey Hanley, Benjamin James Boyd, Thomas Rades, S Hook

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Swelling and phase behaviour of phytantriol and glyrecyl monooleate (GMO) matrices with varying water loadings were investigated. Release of a model protein, FITC-Ova was subsequently examined. Polarised light microscopy and small angle X-ray scattering analysis showed that the addition of FITC-Ova only altered the liquid crystalline structure of phytantriol matrices as both low and high loading reflected that of the binary system. Addition of FITC-Ova to GMO matrices also altered the liquid crystalline structure when compared to the respective binary system at low but not at high loading. All samples analysed after the release study had transformed to the reverse hexagonal phase. Swelling studies revealed a faster and more extensive swelling of GMO when compared to phytantriol. Release of FTIC-Ova from phytantriol matrices was faster and occurred to a greater extent most likely due to the conversion of GMO matrices into the reverse hexagonal phase. No effect on release as a function of initial water content was observed for either lipid. We have confirmed that phytantriol based liquid crystalline matrices can sustain the release of a hydrophilic protein, suggesting its suitability as a potential sustained antigen-delivery system.
Original languageEnglish
Pages (from-to)4191 - 4204
Number of pages14
JournalJournal of Pharmaceutical Sciences
Issue number11
Publication statusPublished - 2009

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