Limited Phenotypic and Functional Plasticity of Influenza Virus-Specific Memory CD8+ T Cells during Activation in an Alternative Cytokine Environment

Kim L. Harland, Annette Fox, Simone Nüssing, Luca Hensen, Katherine Kedzierska, Stephen J. Turner, Anne Kelso

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Naive CD8+ T cells show phenotypic, functional, and epigenetic plasticity, enabling differentiation into distinct cellular states. However, whether memory CD8+ T cells demonstrate similar flexibility upon recall is poorly understood. We investigated the potential of influenza A virus (IAV)-specific memory CD8+ T cells from mice to alter their phenotype and function in response to reactivation in the presence of IL-4 and anti-IFN-γ Ab (type 2 conditions). Compared with naive CD8+ T cells, only a small proportion of IAV-specific memory T cells exhibited phenotypic and functional plasticity after clonal activation under type 2 conditions. The potential for modulation of cell-surface phenotype (CD8α expression) was associated with specific epigenetic changes at the Cd8a locus, was greater in central memory T cells than effector memory T cells, and was observed in endogenous memory cells of two TCR specificities. Using a novel technique for intracellular cytokine staining of small clonal populations, we showed that IAV-specific memory CD8+ T cells reactivated under type 2 conditions displayed robust IFN-γ expression and, unlike naive CD8+ T cells activated under type 2 conditions, produced little IL-4 protein. Secondary activation of memory cells under type 2 conditions increased GATA-3 levels with minimal change in T-bet levels. These data suggest that a small population of memory cells, especially central memory T cells, exhibits plasticity; however, most IAV-specific memory CD8+ T cells resist reprogramming upon reactivation and retain the functional state established during priming.

Original languageEnglish
Pages (from-to)3282-3293
Number of pages12
JournalJournal of Immunology
Volume201
Issue number11
DOIs
Publication statusPublished - 1 Dec 2018

Cite this

Harland, Kim L. ; Fox, Annette ; Nüssing, Simone ; Hensen, Luca ; Kedzierska, Katherine ; Turner, Stephen J. ; Kelso, Anne. / Limited Phenotypic and Functional Plasticity of Influenza Virus-Specific Memory CD8+ T Cells during Activation in an Alternative Cytokine Environment. In: Journal of Immunology. 2018 ; Vol. 201, No. 11. pp. 3282-3293.
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Limited Phenotypic and Functional Plasticity of Influenza Virus-Specific Memory CD8+ T Cells during Activation in an Alternative Cytokine Environment. / Harland, Kim L.; Fox, Annette; Nüssing, Simone; Hensen, Luca; Kedzierska, Katherine; Turner, Stephen J.; Kelso, Anne.

In: Journal of Immunology, Vol. 201, No. 11, 01.12.2018, p. 3282-3293.

Research output: Contribution to journalArticleResearchpeer-review

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