Lim1/LIM1 is expressed in developing and adult mouse and human endometrium

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Lim1 encodes a homeodomain transcription factor required for head, kidney and female reproductive tract development in the murine embryo. Recently, Lim1 expression was documented in several adult murine and human organs. In the developing female reproductive tract, Lim1 expression was first detected in the Mullerian ducts. Using immunofluorescence, we detected LIM1 expression in a developmental model of human female reproductive tract which was established by recombination of neonatal uterine mesenchyme with human embryonic stem cells. In addition, we report a dynamic expression of Lim1/LIM1 in neonatal and adult mouse, and adult human endometrial epithelium and stroma as revealed by immunofluorescence and quantitative real-time polymerase chain reaction. LIM1 expression was also observed in several endometrial epithelial cancer cell lines (ECC-1, Ishikawa, and HEC1A). Furthermore, we found that Activin A significantly upregulated LIM1 mRNA expression in ECC-1 cells. These studies demonstrate previously unreported Lim1/LIM1 expression in neonatal, adult mouse and human endometrium suggesting Lim1/LIM1 may have a role in endometrial development and remodelling.
Original languageEnglish
Pages (from-to)527 - 536
Number of pages10
JournalHistochemistry and Cell Biology
Volume137
Issue number4
DOIs
Publication statusPublished - 2012

Cite this

@article{a6c355a7659347ab960da08d3d32b0e6,
title = "Lim1/LIM1 is expressed in developing and adult mouse and human endometrium",
abstract = "Lim1 encodes a homeodomain transcription factor required for head, kidney and female reproductive tract development in the murine embryo. Recently, Lim1 expression was documented in several adult murine and human organs. In the developing female reproductive tract, Lim1 expression was first detected in the Mullerian ducts. Using immunofluorescence, we detected LIM1 expression in a developmental model of human female reproductive tract which was established by recombination of neonatal uterine mesenchyme with human embryonic stem cells. In addition, we report a dynamic expression of Lim1/LIM1 in neonatal and adult mouse, and adult human endometrial epithelium and stroma as revealed by immunofluorescence and quantitative real-time polymerase chain reaction. LIM1 expression was also observed in several endometrial epithelial cancer cell lines (ECC-1, Ishikawa, and HEC1A). Furthermore, we found that Activin A significantly upregulated LIM1 mRNA expression in ECC-1 cells. These studies demonstrate previously unreported Lim1/LIM1 expression in neonatal, adult mouse and human endometrium suggesting Lim1/LIM1 may have a role in endometrial development and remodelling.",
author = "Ping Ye and Jemma Evans and Caroline Gargett",
year = "2012",
doi = "10.1007/s00418-011-0909-9",
language = "English",
volume = "137",
pages = "527 -- 536",
journal = "Histochemistry and Cell Biology",
issn = "0948-6143",
publisher = "Springer-Verlag London Ltd.",
number = "4",

}

Lim1/LIM1 is expressed in developing and adult mouse and human endometrium. / Ye, Ping; Evans, Jemma; Gargett, Caroline.

In: Histochemistry and Cell Biology, Vol. 137, No. 4, 2012, p. 527 - 536.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Lim1/LIM1 is expressed in developing and adult mouse and human endometrium

AU - Ye, Ping

AU - Evans, Jemma

AU - Gargett, Caroline

PY - 2012

Y1 - 2012

N2 - Lim1 encodes a homeodomain transcription factor required for head, kidney and female reproductive tract development in the murine embryo. Recently, Lim1 expression was documented in several adult murine and human organs. In the developing female reproductive tract, Lim1 expression was first detected in the Mullerian ducts. Using immunofluorescence, we detected LIM1 expression in a developmental model of human female reproductive tract which was established by recombination of neonatal uterine mesenchyme with human embryonic stem cells. In addition, we report a dynamic expression of Lim1/LIM1 in neonatal and adult mouse, and adult human endometrial epithelium and stroma as revealed by immunofluorescence and quantitative real-time polymerase chain reaction. LIM1 expression was also observed in several endometrial epithelial cancer cell lines (ECC-1, Ishikawa, and HEC1A). Furthermore, we found that Activin A significantly upregulated LIM1 mRNA expression in ECC-1 cells. These studies demonstrate previously unreported Lim1/LIM1 expression in neonatal, adult mouse and human endometrium suggesting Lim1/LIM1 may have a role in endometrial development and remodelling.

AB - Lim1 encodes a homeodomain transcription factor required for head, kidney and female reproductive tract development in the murine embryo. Recently, Lim1 expression was documented in several adult murine and human organs. In the developing female reproductive tract, Lim1 expression was first detected in the Mullerian ducts. Using immunofluorescence, we detected LIM1 expression in a developmental model of human female reproductive tract which was established by recombination of neonatal uterine mesenchyme with human embryonic stem cells. In addition, we report a dynamic expression of Lim1/LIM1 in neonatal and adult mouse, and adult human endometrial epithelium and stroma as revealed by immunofluorescence and quantitative real-time polymerase chain reaction. LIM1 expression was also observed in several endometrial epithelial cancer cell lines (ECC-1, Ishikawa, and HEC1A). Furthermore, we found that Activin A significantly upregulated LIM1 mRNA expression in ECC-1 cells. These studies demonstrate previously unreported Lim1/LIM1 expression in neonatal, adult mouse and human endometrium suggesting Lim1/LIM1 may have a role in endometrial development and remodelling.

UR - http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=22231913

U2 - 10.1007/s00418-011-0909-9

DO - 10.1007/s00418-011-0909-9

M3 - Article

VL - 137

SP - 527

EP - 536

JO - Histochemistry and Cell Biology

JF - Histochemistry and Cell Biology

SN - 0948-6143

IS - 4

ER -