Light-sheet in a µtas for single cell imaging

Research output: Chapter in Book/Report/Conference proceedingConference PaperOther

Abstract

The miniaturization of Light-Sheet Fluorescence Microcopy and its integration with a microfluidic system for sample delivery and control is demonstrated in this abstract. A micro-optical system has been designed and fabricated to generate 1 µm thickness light-sheets using air-cavities on PDMS as cylindrical micro-lenses. The material of these micro-lenses eases the coupling to microfluidic devices as they can be monolithically fabricated together. This device enables the high-throughput 3D imaging of single live cells with sub-cellular resolution. This new technology has the potential to change how we image suspension cells and in the future can be easily adopted by many cell biologists.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages809-810
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - 2020
EventInternational Conference on Miniaturized Systems for Chemistry and Life Sciences 2020 - Virtual, Online, United States of America
Duration: 4 Oct 20209 Oct 2020
Conference number: 24th
https://microtas2020.org (Website)

Conference

ConferenceInternational Conference on Miniaturized Systems for Chemistry and Life Sciences 2020
Abbreviated titleMicroTAS 2020
CountryUnited States of America
CityVirtual, Online
Period4/10/209/10/20
Internet address

Keywords

  • Flow cytometry
  • Light-Sheet Fluorescence Microscopy on a Chip
  • Single cell 3D analysis

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