Abstract
The localization of [3H]rauwolscine binding to microscope slide mounted sections of rat kidney has been examined using the technique of in vitro labelling and autoradiography. Binding to sections equilibrated within 60 min and was reversible following the addition of 10 μM phentolamine. Saturation studies revealed a single population of high affinity (KD 4.27 nM) non-interacting sites (nH 0.97) with a density of 11.1 fmol/section. Stereoselectivity was observed with respect to the isomers of noradrenaline and the relative affinity of a series of α-adrenoceptor antagonists suggested binding to α2-adrenoceptors. Autoradiographic studies using 3H-Ultrofilm showed that the binding is largely confined to the renal cortex. More detailed studies using emulsion coated coverslips indicates that the major concentration of binding sites is over the proximal tubules. This study provides evidence that α2-adrenoceptors, known to be coupled in an inhibitory fashion to renal adenylate cyclase, are highly localized to particular structures in the kidney.
| Original language | English |
|---|---|
| Pages (from-to) | 271-278 |
| Number of pages | 8 |
| Journal | European Journal of Pharmacology |
| Volume | 116 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 22 Oct 1985 |
Keywords
- Autoradiography
- Rat kidney
- [H]Rauwolscine
- α-Adrenoceptors
Cite this
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Light microscopic autoradiography of the distribution of [3H]rauwolscine binding to α2-adrenoceptors in rat kidney. / Stephenson, Jennifer A.; Summers, Roger J.
In: European Journal of Pharmacology, Vol. 116, No. 3, 22.10.1985, p. 271-278.Research output: Contribution to journal › Article › Research › peer-review
TY - JOUR
T1 - Light microscopic autoradiography of the distribution of [3H]rauwolscine binding to α2-adrenoceptors in rat kidney
AU - Stephenson, Jennifer A.
AU - Summers, Roger J.
PY - 1985/10/22
Y1 - 1985/10/22
N2 - The localization of [3H]rauwolscine binding to microscope slide mounted sections of rat kidney has been examined using the technique of in vitro labelling and autoradiography. Binding to sections equilibrated within 60 min and was reversible following the addition of 10 μM phentolamine. Saturation studies revealed a single population of high affinity (KD 4.27 nM) non-interacting sites (nH 0.97) with a density of 11.1 fmol/section. Stereoselectivity was observed with respect to the isomers of noradrenaline and the relative affinity of a series of α-adrenoceptor antagonists suggested binding to α2-adrenoceptors. Autoradiographic studies using 3H-Ultrofilm showed that the binding is largely confined to the renal cortex. More detailed studies using emulsion coated coverslips indicates that the major concentration of binding sites is over the proximal tubules. This study provides evidence that α2-adrenoceptors, known to be coupled in an inhibitory fashion to renal adenylate cyclase, are highly localized to particular structures in the kidney.
AB - The localization of [3H]rauwolscine binding to microscope slide mounted sections of rat kidney has been examined using the technique of in vitro labelling and autoradiography. Binding to sections equilibrated within 60 min and was reversible following the addition of 10 μM phentolamine. Saturation studies revealed a single population of high affinity (KD 4.27 nM) non-interacting sites (nH 0.97) with a density of 11.1 fmol/section. Stereoselectivity was observed with respect to the isomers of noradrenaline and the relative affinity of a series of α-adrenoceptor antagonists suggested binding to α2-adrenoceptors. Autoradiographic studies using 3H-Ultrofilm showed that the binding is largely confined to the renal cortex. More detailed studies using emulsion coated coverslips indicates that the major concentration of binding sites is over the proximal tubules. This study provides evidence that α2-adrenoceptors, known to be coupled in an inhibitory fashion to renal adenylate cyclase, are highly localized to particular structures in the kidney.
KW - Autoradiography
KW - Rat kidney
KW - [H]Rauwolscine
KW - α-Adrenoceptors
UR - http://www.scopus.com/inward/record.url?scp=0022344041&partnerID=8YFLogxK
U2 - 10.1016/0014-2999(85)90162-1
DO - 10.1016/0014-2999(85)90162-1
M3 - Article
VL - 116
SP - 271
EP - 278
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 3
ER -