Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration

Daniel Čapek, Michael Smutny, Alexandra-Madelaine Tichy, Maurizio Morri, Harald Janovjak, Carl Philipp Heisenberg

Research output: Contribution to journalArticleResearchpeer-review

9 Citations (Scopus)

Abstract

Non-canonical Wnt signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives cell polarization in epithelial tissues, it remains unclear whether such instructive activity is also critical for directed mesenchymal cell migration. Here, we developed a light-activated version of the non-canonical Wnt receptor Frizzled 7 (Fz7) to analyze how restricted activation of non-canonical Wnt signaling affects directed anterior axial mesendoderm (prechordal plate, ppl) cell migration within the zebrafish gastrula. We found that Fz7 signaling is required for ppl cell protrusion formation and migration and that spatiotemporally restricted ectopic activation is capable of redirecting their migration. Finally, we show that uniform activation of Fz7 signaling in ppl cells fully rescues defective directed cell migration in fz7 mutant embryos. Together, our findings reveal that in contrast to the situation in epithelial cells, non-canonical Wnt signaling functions permissively rather than instructively in directed mesenchymal cell migration during gastrulation.

Original languageEnglish
Article numbere42093
Number of pages24
JournaleLife
Volume8
DOIs
Publication statusPublished - 16 Jan 2019

Keywords

  • cell biology
  • cell migration
  • developmental biology
  • optogenetics
  • planar cell polarity
  • polarity
  • protrusion formation
  • Wnt
  • zebrafish

Cite this

Čapek, Daniel ; Smutny, Michael ; Tichy, Alexandra-Madelaine ; Morri, Maurizio ; Janovjak, Harald ; Heisenberg, Carl Philipp. / Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration. In: eLife. 2019 ; Vol. 8.
@article{90ac20832e9b49298b3a0a62b5680965,
title = "Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration",
abstract = "Non-canonical Wnt signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives cell polarization in epithelial tissues, it remains unclear whether such instructive activity is also critical for directed mesenchymal cell migration. Here, we developed a light-activated version of the non-canonical Wnt receptor Frizzled 7 (Fz7) to analyze how restricted activation of non-canonical Wnt signaling affects directed anterior axial mesendoderm (prechordal plate, ppl) cell migration within the zebrafish gastrula. We found that Fz7 signaling is required for ppl cell protrusion formation and migration and that spatiotemporally restricted ectopic activation is capable of redirecting their migration. Finally, we show that uniform activation of Fz7 signaling in ppl cells fully rescues defective directed cell migration in fz7 mutant embryos. Together, our findings reveal that in contrast to the situation in epithelial cells, non-canonical Wnt signaling functions permissively rather than instructively in directed mesenchymal cell migration during gastrulation.",
keywords = "cell biology, cell migration, developmental biology, optogenetics, planar cell polarity, polarity, protrusion formation, Wnt, zebrafish",
author = "Daniel Čapek and Michael Smutny and Alexandra-Madelaine Tichy and Maurizio Morri and Harald Janovjak and Heisenberg, {Carl Philipp}",
year = "2019",
month = "1",
day = "16",
doi = "10.7554/eLife.42093",
language = "English",
volume = "8",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications, Ltd",

}

Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration. / Čapek, Daniel; Smutny, Michael; Tichy, Alexandra-Madelaine; Morri, Maurizio; Janovjak, Harald; Heisenberg, Carl Philipp.

In: eLife, Vol. 8, e42093, 16.01.2019.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Light-activated Frizzled7 reveals a permissive role of non-canonical wnt signaling in mesendoderm cell migration

AU - Čapek, Daniel

AU - Smutny, Michael

AU - Tichy, Alexandra-Madelaine

AU - Morri, Maurizio

AU - Janovjak, Harald

AU - Heisenberg, Carl Philipp

PY - 2019/1/16

Y1 - 2019/1/16

N2 - Non-canonical Wnt signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives cell polarization in epithelial tissues, it remains unclear whether such instructive activity is also critical for directed mesenchymal cell migration. Here, we developed a light-activated version of the non-canonical Wnt receptor Frizzled 7 (Fz7) to analyze how restricted activation of non-canonical Wnt signaling affects directed anterior axial mesendoderm (prechordal plate, ppl) cell migration within the zebrafish gastrula. We found that Fz7 signaling is required for ppl cell protrusion formation and migration and that spatiotemporally restricted ectopic activation is capable of redirecting their migration. Finally, we show that uniform activation of Fz7 signaling in ppl cells fully rescues defective directed cell migration in fz7 mutant embryos. Together, our findings reveal that in contrast to the situation in epithelial cells, non-canonical Wnt signaling functions permissively rather than instructively in directed mesenchymal cell migration during gastrulation.

AB - Non-canonical Wnt signaling plays a central role for coordinated cell polarization and directed migration in metazoan development. While spatiotemporally restricted activation of non-canonical Wnt-signaling drives cell polarization in epithelial tissues, it remains unclear whether such instructive activity is also critical for directed mesenchymal cell migration. Here, we developed a light-activated version of the non-canonical Wnt receptor Frizzled 7 (Fz7) to analyze how restricted activation of non-canonical Wnt signaling affects directed anterior axial mesendoderm (prechordal plate, ppl) cell migration within the zebrafish gastrula. We found that Fz7 signaling is required for ppl cell protrusion formation and migration and that spatiotemporally restricted ectopic activation is capable of redirecting their migration. Finally, we show that uniform activation of Fz7 signaling in ppl cells fully rescues defective directed cell migration in fz7 mutant embryos. Together, our findings reveal that in contrast to the situation in epithelial cells, non-canonical Wnt signaling functions permissively rather than instructively in directed mesenchymal cell migration during gastrulation.

KW - cell biology

KW - cell migration

KW - developmental biology

KW - optogenetics

KW - planar cell polarity

KW - polarity

KW - protrusion formation

KW - Wnt

KW - zebrafish

UR - http://www.scopus.com/inward/record.url?scp=85061155522&partnerID=8YFLogxK

U2 - 10.7554/eLife.42093

DO - 10.7554/eLife.42093

M3 - Article

VL - 8

JO - eLife

JF - eLife

SN - 2050-084X

M1 - e42093

ER -