TY - JOUR
T1 - Light-activated chimeric GPCRs
T2 - limitations and opportunities
AU - Tichy, Alexandra-Madelaine
AU - Gerrard, Elliot J.
AU - Sexton, Patrick M.
AU - Janovjak, Harald
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Light-activated chimeric GPCRs, termed OptoXRs, can elicit cell signalling responses with the high spatial and temporal precision of light. In recent years, an expanding OptoXR toolkit has been applied to, for example, dissect neural circuits in awake rodents, guide cell migration during vertebrate development and even restore visual responses in a rodent model of blindness. OptoXRs have been further developed through incorporation of highly sensitive photoreceptor domains and a plethora of signalling modules. The availability of new high-resolution structures of GPCRs and a deeper understanding of GPCR function allows critically revisitation of the design of OptoXRs. Next-generation OptoXRs will build on advances in structural biology, receptor function and photoreceptor diversity to manipulate GPCR signalling with unprecedented accuracy and precision.
AB - Light-activated chimeric GPCRs, termed OptoXRs, can elicit cell signalling responses with the high spatial and temporal precision of light. In recent years, an expanding OptoXR toolkit has been applied to, for example, dissect neural circuits in awake rodents, guide cell migration during vertebrate development and even restore visual responses in a rodent model of blindness. OptoXRs have been further developed through incorporation of highly sensitive photoreceptor domains and a plethora of signalling modules. The availability of new high-resolution structures of GPCRs and a deeper understanding of GPCR function allows critically revisitation of the design of OptoXRs. Next-generation OptoXRs will build on advances in structural biology, receptor function and photoreceptor diversity to manipulate GPCR signalling with unprecedented accuracy and precision.
UR - http://www.scopus.com/inward/record.url?scp=85067176020&partnerID=8YFLogxK
U2 - 10.1016/j.sbi.2019.05.006
DO - 10.1016/j.sbi.2019.05.006
M3 - Review Article
AN - SCOPUS:85067176020
SN - 0959-440X
VL - 57
SP - 196
EP - 203
JO - Current Opinion in Structural Biology
JF - Current Opinion in Structural Biology
ER -