Levels of regulatory B cells do not predict serological responses to hepatitis B vaccine

Maria Bolther, Karen Lise Dahl Andersen, Martin Tolstrup, Kumar Visvanathan, Ian Woolley, Narelle Skinner, Rosemary Millen, Nadia Warner, Lars Østergaard, Søren Jensen-Fangel

Research output: Contribution to journalArticleResearchpeer-review

Abstract

This study investigated the immunomodulatory influence of IL10 producing B regulatory cells, Bregs (CD19+CD24hiCD38hi) to standard Twinrix® vaccination. We also investigated HBsAg specific T-cell mediated IFN-γ responses to Twinrix® which in theory could provide effective immunity despite low anti-HBs titer. A total of 309 hepatitis B negative health care students and workers completed a standard Twinrix® vaccination schedule (0, 1 and 6 months). Depending on the vaccination response the participants were divided in to non-, low- and high responders according to anti-HBs titer (<10, <100 and >1000 mIU/mL respectively) two months after completed vaccination schedule. Blood samples from baseline and after vaccination from all non- and low-responders (23 participants) and the same number of high-responders were used for flow cytometric analyses of IL10 producing Bregs and T-cell mediated IFN-γ responses. A decrease in levels of IL10 producing Bregs was observed after vaccination in high responders compared to non- and low-responders. Compiling non-and low-responders against high-responders showed a lower T-cell mediated IFN-γ response at baseline in non-and low-responders when stimulated with Engerix® vaccine. In contrary no positive correlation between IL10 producing Bregs or IFN-γ positive T-cells and anti-HBs titer was observed. Hence this study cannot prove that levels of IL10 producing Bregs or IFN-γ positive T cell affect HBV vaccine response.

Original languageEnglish
Pages (from-to)1483-1488
Number of pages6
JournalHuman Vaccines & Immunotherapeutics
Volume14
Issue number6
DOIs
Publication statusPublished - 11 Apr 2018

Keywords

  • clinical
  • flow cytometry
  • hepatitis
  • Hepatitis B
  • Interferon γ
  • Interleukin 10
  • non-responders
  • Twinrix
  • vaccinology

Cite this

Bolther, M., Andersen, K. L. D., Tolstrup, M., Visvanathan, K., Woolley, I., Skinner, N., ... Jensen-Fangel, S. (2018). Levels of regulatory B cells do not predict serological responses to hepatitis B vaccine. Human Vaccines & Immunotherapeutics, 14(6), 1483-1488. https://doi.org/10.1080/21645515.2018.1441653
Bolther, Maria ; Andersen, Karen Lise Dahl ; Tolstrup, Martin ; Visvanathan, Kumar ; Woolley, Ian ; Skinner, Narelle ; Millen, Rosemary ; Warner, Nadia ; Østergaard, Lars ; Jensen-Fangel, Søren. / Levels of regulatory B cells do not predict serological responses to hepatitis B vaccine. In: Human Vaccines & Immunotherapeutics. 2018 ; Vol. 14, No. 6. pp. 1483-1488.
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abstract = "This study investigated the immunomodulatory influence of IL10 producing B regulatory cells, Bregs (CD19+CD24hiCD38hi) to standard Twinrix{\circledR} vaccination. We also investigated HBsAg specific T-cell mediated IFN-γ responses to Twinrix{\circledR} which in theory could provide effective immunity despite low anti-HBs titer. A total of 309 hepatitis B negative health care students and workers completed a standard Twinrix{\circledR} vaccination schedule (0, 1 and 6 months). Depending on the vaccination response the participants were divided in to non-, low- and high responders according to anti-HBs titer (<10, <100 and >1000 mIU/mL respectively) two months after completed vaccination schedule. Blood samples from baseline and after vaccination from all non- and low-responders (23 participants) and the same number of high-responders were used for flow cytometric analyses of IL10 producing Bregs and T-cell mediated IFN-γ responses. A decrease in levels of IL10 producing Bregs was observed after vaccination in high responders compared to non- and low-responders. Compiling non-and low-responders against high-responders showed a lower T-cell mediated IFN-γ response at baseline in non-and low-responders when stimulated with Engerix{\circledR} vaccine. In contrary no positive correlation between IL10 producing Bregs or IFN-γ positive T-cells and anti-HBs titer was observed. Hence this study cannot prove that levels of IL10 producing Bregs or IFN-γ positive T cell affect HBV vaccine response.",
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Bolther, M, Andersen, KLD, Tolstrup, M, Visvanathan, K, Woolley, I, Skinner, N, Millen, R, Warner, N, Østergaard, L & Jensen-Fangel, S 2018, 'Levels of regulatory B cells do not predict serological responses to hepatitis B vaccine', Human Vaccines & Immunotherapeutics, vol. 14, no. 6, pp. 1483-1488. https://doi.org/10.1080/21645515.2018.1441653

Levels of regulatory B cells do not predict serological responses to hepatitis B vaccine. / Bolther, Maria; Andersen, Karen Lise Dahl; Tolstrup, Martin; Visvanathan, Kumar; Woolley, Ian; Skinner, Narelle; Millen, Rosemary; Warner, Nadia; Østergaard, Lars; Jensen-Fangel, Søren.

In: Human Vaccines & Immunotherapeutics, Vol. 14, No. 6, 11.04.2018, p. 1483-1488.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Andersen, Karen Lise Dahl

AU - Tolstrup, Martin

AU - Visvanathan, Kumar

AU - Woolley, Ian

AU - Skinner, Narelle

AU - Millen, Rosemary

AU - Warner, Nadia

AU - Østergaard, Lars

AU - Jensen-Fangel, Søren

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KW - Interleukin 10

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