Leptospiral LruA is required for virulence and modulates an interaction with mammalian apolipoprotein AI

Leptospirosis is a worldwide zoonosis caused by spirochetes of the genus Leptospira. While understanding of pathogenesis remains limited, the development of mutagenesis in Leptospira has provided a powerful tool for identifying novel virulence factors. LruA is a lipoprotein that has been implicated in leptospiral uveitis as a target of the immune response. In this study, two lruA mutants, M754 and M765, generated by transposon mutagenesis from Leptospira interrogans serovar Manilae, were characterized. In M754 the transposon inserted in the middle of lruA resulting in no detectable expression of LruA. In M765 the transposon inserted towards the 3 end of the gene, resulting in expression of a truncated protein. LruA was demonstrated to be on the cell surface in M765 and wild-type (WT). M754, but not M765, was attenuated in a hamster model of acute infection. A search for differential binding to human serum proteins identified a serum protein of around 30 kDa bound to wild type and the LruA deletion (M754), but not to the LruA truncation (M765). Two dimensional separation of proteins from leptospiral cells incubated with guinea pig serum identified the 28 kDa Apolipoprotein A-I (ApoA-I) as a major mammalian serum protein that binds Leptospira in vitro. Interestingly, M754 (with no detectable LruA) bound more ApoA-I than the LruA -expressing strains Manilae wild-type and M765. Our data thus identify LruA as a surface-exposed leptospiral virulence factor that contributes to leptospiral pathogenesis, possibly by modulating cellular interactions with serum protein ApoA-I.