Lentiviral vectors containing an enhancer-less ubiquitously acting chromatin opening element (UCOE) provide highly reproducible and stable transgene expression in hematopoietic cells

Fang Zhang, Susannah Thornhill, Steven Howe, Meera Senthuren, Axel Schambach, Joanna Sinclair, Christine Kinnon, Bobby Gaspar, Michael Antoniou, Adrian Thrasher

Research output: Contribution to journalArticleResearchpeer-review

129 Citations (Scopus)

Abstract

Ubiquitously acting chromatin opening elements (UCOEs) consist of methylation-free CpG islands encompassing dual divergently transcribed promoters of housekeeping genes that have been shown to confer resistance to transcriptional silencing and to produce consistent and stable transgene expression in tissue culture systems.
Original languageEnglish
Pages (from-to)1448 - 1457
Number of pages10
JournalBlood
Volume110
DOIs
Publication statusPublished - 2007

Cite this