Legumain targeting peptide conjugated fluorescent porous silicon nanoparticles for breast cancer imaging

Jayasree S. Kanathasan, Varghese Swamy, Uma Devi Palanisamy, Ammu Kutty G. K. Radhakrishnan

Research output: Chapter in Book/Report/Conference proceedingConference PaperResearchpeer-review

Abstract

Porous silicon (PSi) with a suite of most desirable biomaterial properties has attracted great attention as a multifunctional nanoplatform for bioimaging and drug delivery. Various surface functionalization treatments have been reported for PSi to use as an active tumor cell targeting nanovector. In this study, we investigated surface functionalization treatments using a peptide that is specific to the emerging biomarker legumain. The PSi nanoparticles were coated with dextran and subsequently two types of legumain targeting peptide, Y-shaped and linear chain, were conjugated to produce the functionalized PSi. The functionalized (ligand-conjugated) PSi materials were characterized for morphology, size, functional groups, and fluorescence response using electron and fluorescence microscopy and vibrational spectroscopy techniques. Fluorescence microscopy imaging with two excitation wavelengths (450 nm and 600 nm) suggests comparable fluorescence response of the conjugated PSi to “bare” PSi and the suitability of the PSi functionalized with peptide for bioimaging.
Original languageEnglish
Title of host publication7th Forum on New Materials
Subtitle of host publicationPart F
EditorsPietro Vincenzini
Place of PublicationSwiterland
PublisherTrans Tech Publications
Chapter3
Pages45-50
Number of pages6
DOIs
Publication statusPublished - 2016
EventMedical Applications of Novel Biomaterials and Nanotechnology 2016 - Perugia, Italy
Duration: 5 Jun 20169 Jun 2016
https://main.scientific.net/book/7th-forum-on-new-materials-part-f/978-3-0357-3125-5/ebook

Publication series

NameAdvances in Science and Technology
PublisherTrans Tech Publications
Volume102
ISSN (Print)1662-0356

Conference

ConferenceMedical Applications of Novel Biomaterials and Nanotechnology 2016
Country/TerritoryItaly
CityPerugia
Period5/06/169/06/16
Internet address

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