LC-MS/MS determination of colistin in Mueller-Hinton broth for in vitro pharmacodynamic studies

Miao Zhao, Yu-Ran Cao, Bei-Ning Guo, Xiao-Jie Wu, Jian Li, Jing Zhang

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Abstract

A rapid and simple method was developed and validated for the determination of colistin A and B in Mueller-Hinton broth using LC-tandem MS. Both analyte and internal standard (IS) (polymyxin B1) were determined using ESI. The MS data were obtained via the selected reaction monitoring in positive-ion mode. A linear regression (weighted 1/concentration) was used to fit calibration curves over the concentration range of 0.0241-2.41 ?g ml-1 for colistin A and 0.0439-4.39 ?g ml-1 for colistin B. No interference peaks were found in the blank Mueller-Hinton broth tested. Inter-and intraday precision and accuracy were within 85-115 (coefficient of variation). Colistin was stable in the autosampler for at least 24 h at 4 ?C and in Mueller-Hinton broth for at least 120 h at 35 ?C. This assay has been successfully used to determine colistin A and B in Mueller-Hinton broth for in vitro pharmacodynamic model studies. Accurate determination of colistin in bacterial growth medium has a vital role in the studies examining dosage regimen of and bacterial resistance to colistin.
Original languageEnglish
Pages (from-to)825 - 829
Number of pages5
JournalJournal of Antibiotics
Volume67
Issue number12
DOIs
Publication statusPublished - 2014

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