Late passage human fibroblasts induced to pluripotency are capable of directed neuronal differentiation

Jun Liu, Huseyin Sumer, Jessie Leung, Kyle Upton, Mirella Dottori, Alice Pebay, Paul Verma

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    14 Citations (Scopus)


    It is possible to generate induced pluripotent stem (iPS) cells from mouse and human somatic cells by ectopic expression of defined sets of transcription factors. However, the recommendation that somatic cells should be utilized at early passages for induced reprogramming limits their therapeutic application. Here we report successful reprogramming of human fibroblasts after more than 20 passages in vitro, to a pluripotent state with four transcription factors: Oct4, Sox2, Klf4, and c-Myc. The late passage-derived human iPS cells resemble human embryonic stem cells in morphology, cell surface antigens, pluripotent gene expression profiles, and epigenetic states. Moreover, these iPS cells differentiate into cell types representative of the three germ layers in teratomas in vivo, and directed neuronal differentiation in vitro.
    Original languageEnglish
    Pages (from-to)193 - 203
    Number of pages11
    JournalCell Transplantation
    Issue number2
    Publication statusPublished - 2011

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