Laser microbeam-induced fixation for electronmicroscopy: Visualization of transient developmental features in nematode embryos

T. S. Cole, E. Schierenberg

Research output: Contribution to journalArticleResearchpeer-review

14 Citations (Scopus)

Abstract

In order to study development of embryos of Caenorhabditis elegans at an ultrastructural level, a new method of fixation has been developed. With a laser microbeam coupled to a microscope the impermeable eggshell is punctured to allow penetration of the fixative. At specific stages of embryogenesis further development can be arrested at will under visual control. As fixation occurs instantaneously, transient events (e.g. different phases of mitosis and cytokinesis) can be visualized.

Original languageEnglish
Pages (from-to)1046-1048
Number of pages3
JournalExperientia
Volume42
Issue number9
DOIs
Publication statusPublished - 1 Sep 1986
Externally publishedYes

Keywords

  • Electronmicroscopy
  • embryogenesis
  • germline
  • laser
  • mitosis

Cite this