Laser-mediated microlesions in mouse neocortex to investigate neuronal degeneration and regeneration

Johanna Jackson, Alison J Canty, Lieven Huang, Vincenzo De Paola

Research output: Contribution to journalArticleResearchpeer-review

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Abstract

In vivo two-photon (2P) imaging enables neural circuitry to be repeatedly visualized in both normal conditions and following trauma. This protocol describes how laser-mediated neuronal microlesions can be created in the cerebral cortex using an ultrafast laser without causing a significant inflammatory reaction or compromising the blood-brain barrier. Furthermore, directives are provided for the acute and chronic in vivo imaging of the lesion site, as well as for post-hoc analysis of the lesion site in fixed tissue, which can be correlated with the live imaging phase. (c) 2015 by John Wiley Sons, Inc.
Original languageEnglish
Pages (from-to)1 - 17
Number of pages17
JournalCurrent Protocols in Neuroscience
Volume73
Issue number2.24
DOIs
Publication statusPublished - 2015

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